纳米珍珠粉对大鼠深ⅱ°烧伤创面愈合的影响

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1、纳米珍珠粉对大鼠深Ⅱ°烧伤创面愈合的影响作者：崔淑芳，郝光荣，汤球，孙伟，曹平【摘要】目的研究纳米珍珠粉对大鼠深Ⅱ°烧伤创面愈合的影响并与微米珍珠粉在烧伤愈合中进行药效比较。方法纳米珍珠粉、微米珍珠粉(0.5g/只)分别与甘油按1∶1配制成混合液。以微米珍珠粉组与自然愈合组作对照，纳米珍珠粉组为实验组。测定大鼠14%深Ⅱ°烫伤后7、15、21天的创面愈合率、创面组织学观察以及皮肤羟脯氨酸的含量。结果纳米珍珠粉组创面愈合率较微米珍珠粉组、自然愈合组高(P＜0.05或P＜0.01)；烧伤后3天各组皮肤羟脯氨酸含量均低于正常(P＜0.01)，纳米珍珠粉组减少程度小于其他组；伤

2、后5天各组HP含量有所恢复，尤以纳米珍珠粉组显著高于自然愈合组及微米珍珠粉组；10天后各组明显升高，而纳米珍珠粉组HP含量的升高差异有非常显著性(P＜0.01)。结论纳米珍珠粉可以促进大鼠深Ⅱ°烧伤创面的愈合，其愈合作用明显高于微米珍珠粉(P＜0.05)。【关键词】纳米珍珠粉；微米珍珠粉；烧伤；羟脯氨酸【Abstract】ObjectiveTostudytheefficiencyparisonbeticropearlpoentofdeepⅡ°skinscaldsinrats.MethodsNanoandmicropearlpoixedicropearlpoalheali

3、nggroupasthecontrol，nanopearlpoentalgroups.>P＜0.05orP＜0.01).Atthe3thdaypost-injury，thecontentofHPineverygroupalgroup(P＜0.01)，eextent，otetheorehigherthanthatofmicronpearlpoicropearlpoentofdeepⅡ°skinscaldsinratsandparethefunctionicronpearlpoethods1.1PearlpoaceuticalCo.，Ltd)，nanoandmicropea

4、rlpoixedalhydroxyprolinedetectionkit(NanjingJianchengbiotechnologyInstitute，ApprovedNumber20040631).1.2ExperimentalanimalsFemaleSDrats(n＝190，bodyentalAnimalCenter，SecondMilitaryMedicalUniversity).Theratsetaboliccagesfor1entalanimal［SCXK(Hu)2002-0006］；qualifiedcertificateforanimalfacility

5、［No.SCXK(Hu)2002-0026］.1.3EstablishmentofdeepⅡ°skinscaldsmodelinratsThemodeladebasedonthetraditionalmethodsetupbyYangTaimEI［1］.Theratsodelinratsicropearlpoalhealinggroup.Innanopearlpoicropearlpoixtureofnano，micropearlpoalhealinggroupentlasted21days.1.5Observations1.5.1stanceTheenthealing

6、percentageandhealingcircumstancee.1.5.2Dermalhydroxyproline(HP)measurementTissueinthethe3groupsofratsonday3，5，10，15and21afterscaldingalratsskinasacontrol.ThedorsalskintheconnectivetissuefollogofeasuredbydermalHPreagentkit.HPcontentg.1.6DatastatisticsandanalysisInputtingthedataintothepute

7、r，toanalyzethedatailarityofgroupdataeandtheenthealingrateTheskinicropearlpoeofnanopearlpoilaritymorethanthatofmicropearlpoenthealingrateismorequickly.(P<0.01，Seetable1fordetails).Tab.1parisonofeanddevelopmenthealingrateofthedifferenttimes(x±s，%)0Note：paredicropearlpo.2