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1、马齿苋总黄酮抗小鼠缺氧作用及其机制研究论文董立巍，王万银，岳义田，李敏【关键词】,马齿苋［摘要］目的：筛选马齿苋抗缺氧作用有效成分，并对筛选出的有效成分进行作用机制的研究。方法：马齿苋抗缺氧作用有效成分筛选：将70只小鼠随机分为总黄酮组、总生物碱组、多糖组、酸性成分组、碱性成分组、人参总皂苷组（阳性对照）和生理盐水组（阴性对照），每组10只。各组小鼠予以相应药物灌胃1周后，进行常压缺氧暴露生存时间实验，筛选出马齿苋抗缺氧作用的有效成分。马齿苋总黄酮抗缺氧机制研究：将90只小鼠随机分为总黄酮组、人参总皂苷组和生理盐水组.freelRNA的表达水平。结果：与生理盐水组比较，马齿苋总黄酮组小鼠缺
2、氧暴露生存时间明显延长；马齿苋总黄酮组小鼠不同缺氧时段肾脏和大脑皮质EPOmRNA的表达水平明显增加，血浆EPO、红细胞计数和血红蛋白含量亦明显升高。结论：总黄酮是马齿苋抗缺氧作用的有效成分，其延长小鼠缺氧生存时间的机制可能是促进EPO的表达以及红细胞和血红蛋白的生成。［关键词］马齿苋;黄酮;缺氧;血红蛋白类;红细胞生成素EffectsofflavonesextractedfromPortulacaoleraceaonabilityofhypoxiatoleranceinmiceanditsmechanismABSTRACTObjective:Toidentifyantihypoxiain
3、gredientsextractedfromPortulacaoleraceaandtofindoutthepossiblemechanismofitsantihypoxiaactions.Methods:Seventymicelydividedintosevengroupsalsaline),ginsenosidestreated,polysaccharidetreated,acidicponentstreated,basicponentstreated,alkaloidstreatedandflavonestreatedgroups,andtheingredientsofpolysac
4、charide,acidicponents,basicponents,alkaloidsandflavonesPortulacaolerace.Themiceineachgroupeofmiceinhypoxicconditionsicealsaline),ginsenosidestreatedandflavonestreatedgroups.Themiceineachofthese3groupsoglobin(Hb)concentrationandhematocrit(HCT)inmiceined.Theplasmaerythropoietin(EPO)levelsofmiceelink
5、edimmunosorbentassay(ELISA)andtherelativevaluesofEPOmRNAinrenaltissueandpalliumofmiceinedbyreversetranscriptasepolymerasechainreaction(RTPCR).Results:ThesurvivaltimeofmiceinhypoxicconditionsinflavonestreatedgroupaEPOlevelandtherelativevaluesofEPOmRNAinrenaltissueandpalliumofmicePortulacaoleraceaar
6、eflavonesandtheantihypoxiaeffectsmaybeobtainedbyimprovingtheexpressionlevelofEPOandacceleratingthegenerationsoferythrocyteandHb.KEY］.第3版.北京:人民卫生出版社,2002.15101512.8PriyadarshiA,PeriyasamyS,BurkeTJ,etal.Effectsofreductionofrenalmassonrenaloxygentensionanderythropoietinproductionintherat［J］.KidneyInt
7、,2002,61(2):542546.9BernaudinM,BellailA,MartiHH,etal.NeuronsandastrocytesexpressEPOmRNA:oxygensensingmechanismsthatinvolvetheredoxstateofthebrain［J］.Glia,2000,30(3):271278.10ChikumaM,MasudaS,KobayashiT,etal.Tissu
