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1、水稻同源异型域转录因子OsHox9的反向遗传学分析艾丽萍 申奥 高志超 李政龙 孙琼琳 栾维江∗(天津师范大学生命科学学院/天津市动植物抗性重点实验室,天津300387;∗通讯联系人,EGmail:lwjzsq@163.com)ReverseGeneticsAnalysisoftheTranscriptionFactorOsHox9,aMemberofHomeoboxFamily,inRiceAILiGping,SHENAo,GAOZhiGchao,LIZhengGlong,SUNQiongGlin,LUANWeiGjiang∗(CollegeofLifeScience,Tianji
2、nNormalUniversity/TianjinKeyLaboratoryofAnimalandPlantResistance,Tianjin300387,China;∗Correspondingauthor,EGmail:lwjzsq@163.com)AILiping,SHENAo,GAOZhichao,etal.ReversegeneticsanalysisofthetranscriptionfactorOsHox9,amemberofhomeoboxfamily,inrice.ChinJRiceSci,2014,28(3):223G228.Abstract:Thehomeob
3、oxtranscriptionfactorsplayimportantrolesinthegrowthanddevelopmentoftheplants,involvedinregulationofcelldifferentiation,thearchitectureofmorphogenesisandresponsetoenvironmentalsignalsinplants.Tostudythefunctionofthesetranscriptionfactorsinrice,weconstructedRNAivectorsofOsHox9andanalyzedthefuncti
4、onofOsHox9bythereversegeneticsmethod.TheresultsshowedthattheheightandtillernumberofRNAitransgenicplantsweredecreasedincomparisonwithwildtypeplants.RealGtimePCRanalysisshowedthatOsHox9expressionlevelwasdownregulatedintransgenicplantswithphenotype.However,theexpressionlevelofOsHox9intransgenicpla
5、ntswithoutphenotypewassimilartowildtypeplants,suggestingthatthephenotypesoftransgenicplantswerecausedbyRNAieffects.Inaddition,wealsoanalyzedtheexpressionpatternofOsHox9indifferentorgansofrice.TheresultsshowedthatOsHox9wasexpressedindifferentorgans,especiallydisplayedhighexpressionlevelinstemapi
6、calmeristemsandyoungpanicles.ToinvestigatethesubcellularlocalizationofOsHox9,weconstructedthefusedvector35S::OsHox9GGFPtointroduceintoleavesoftobacco.ThetransientexpressionresultshowedOsHox9waslocalizedonthecellmembrane.Keywords:rice;OsHox9;RNAi;expressionanalysis艾丽萍,申奥,高志超,等.水稻同源异型域转录因子OsHox9的
7、反向遗传学分析.中国水稻科学,2014,28(3):223G228.摘 要:同源异型域(Homeobox,HB)转录因子对于植物的生长发育具有重要的调控作用,主要涉及细胞分化、形态建成、内外环境信号应答等多个方面.为了研究该转录因子家族成员在水稻中的功能,构建了该家族成员OsHox9基因的RNAi表达载体,利用反向遗传方法分析该基因的功能.与野生型对照植株相比,RNAi转基因植株株高变矮,分蘖数减少.实时PCR表达分析表明OsHox9基因在有表型转基因植株