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1、FoodChemistry111(2008)745–749ContentslistsavailableatScienceDirectFoodChemistryjournalhomepage:www.elsevier.com/locate/foodchemIsolationandcharacterizationofnaturalmelaninderivedfromsilkyfowl(GallusgallusdomesticusBrisson)Si-RuiChen,BinJiang,Jiang-Xia
2、Zheng,Gui-YunXu,Jun-YinLi,NingYang*CollegeofAnimalScienceandTechnologyandStateKeyLaboratoryofAgroBiotechnology,ChinaAgriculturalUniversity,Beijing100193,ChinaarticleinfoabstractArticlehistory:Received17December2007Receivedinrevisedform29February2008Ac
3、cepted22April2008Keywords:MelaninEumelaninSilkyfowlPhysicochemicalpropertyMorphologyWater-insolublemelaninsderivedfromsilkyfowl(SF-melanin)wereisolatedusinganenzymaticextrac-tionprocedure.Theyieldsofthepigmentsfromperiosteum,ovaryortestis,trachea,skin
4、andmuscle,were21.3,13.7,10.2,1.1,and1.0mg/g,respectively,onafreshtissuebasis.Theisolatedpigmentswereidenti-fiedasmelaninsaccordingtotheElectronParamagneticResonanceSpectroscopyspectra.Usingsyntheticmelaninasacalibration,theirphysicochemicalandmorpholog
5、icalpropertieswerefurthercharacterizedbyX-rayphotoelectronspectroscopy(XPS)andadvancedimagingtechnology.ElementalcompositionanalysisbyXPSrevealedthatthemaincomponentoftheSF-melaninwaseumelanin.Themorphologicalstudyshowedthat(1)theSF-melanindisplayedel
6、lipsoidalmelanosomeswhichwasregardedasrepresen-tativeofthenaturalmaterial;and(2)theSF-melaninmaintaineditsnaturalintegrity.Inconclusion,ourenzymaticextractionmethodyieldedhighlypurifiedproductswhilemaintainingthenaturalpropertiesoftheSF-melanin,whichco
7、uldbechemically,physically,morphologically,definedaseumelanin.Ó2008ElsevierLtd.Allrightsreserved.1.IntroductionBlack-bonesilkyfowl(GallusgallusdomesticusBrisson),knownasamarveloftraditionalChinesemedicine,livesuptoitsnamewithsnow-whitesilkyfeatherbutbl
8、ackskin,meatandbones.Asauniquechickenbreed,thesilkyfowl,originatedfromthesouthofChina,isdistinguishedfrombroilerandlayerchickenaccordingtothegenome-widegeneticvariationanalysis(InternationalChickenPolymorphismMapConsortium.,2004).Generally,the
