mirna-135b、mirna-141在结直肠癌中表达水平及其临床意义

《mirna-135b、mirna-141在结直肠癌中表达水平及其临床意义》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、授予单位代码10089学号或申请号15433中国图书分类号R363.2+1HebeiMedicalUniversity硕士学位论文学术学位miRNA-135b、miRNA-141在结直肠癌中表达水平及其临床意义研究生：甄晓月导师：张志勇教授专业：病理学与病理生理学二级学院：唐山市工人医院2018年03月目录中文摘要··········································································································

2、·······1英文摘要·················································································································4英文缩写·················································································································7研究论文miRNA-135b、miRNA

3、-141在结直肠癌中的表达水平及其临床意义前言·················································································································8材料与方法····································································································9结果…………………………………………………

4、………………………………………………14附图···············································································································17附表···············································································································20讨论············

5、···································································································24结论···············································································································26参考文献············································

6、··························································27综述·microRNA在结直肠癌中的研究进展···················································30致谢·······················································································································51个人简历··

7、············································································································52miRNA-135b、miRNA-141在结直肠癌中的表达水平及其临床意义摘要目的：本研究通过检测结直肠癌患者中癌组织以及其对应的上切缘无瘤粘膜组织中microRNA-135b(miR-135b)和microRNA-141(miR-141)的表达，分析其与结直肠癌的临床病理特征之间的关系，及探讨miR-13

8、5b和miR-141的表达在结直肠癌患者中的早期预警、诊断及其在治疗中的价值。方法：1.收集2015年1月到2016年1月唐山工人医院肛肠外科及肿瘤外科，结直肠癌手术患者的新鲜癌组织标本28例；同时收集与其对应的上切缘无瘤粘膜组织28例，迅速将离体后标本置于液氮中，然后存放于-80℃冰箱内以备用；2.将