基于有机纳米功能膜光学生物传感的研究

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1、ABSTRACTRecently，therehasbeenarapidgrowthinthedevelopmentofthehighlyselectiveandsensitiveopticalnanobiosensorsbasedongoldnanoparticlesmodifiedfilmsfordiseasediagnosis，drugdiscovery,proteomicsandmolecularbiology．Takingpolyvinylpyrrolidone(PVP)asprotectiveagentandsodiumcitrateas

2、reducingagent，homogeneousgoldsolswerepreparedbychemicalreductioninthispaper．DL-1，4-dithiothreitol(DTT)wasfirstself-assembledonaflatgoldplateelectrodewithasurfaceareaof5mmx5mm，3-mercaptopropionicacidwaschosentobindwithgoldparticstoformmolecularrecognitionbiosensorsincomparisonw

3、iththatofL—cysteine．ThepreparedgoldnanoparticleswerecharacteredwithUV-VisandTEM．Andtheirassemblingprocessesweretestedbycylicvoltammetry．BrokenthroughthetraditionalcatalysisofEDC—NHS，theEDC-DMAPasanicecatalystofinteractionbetweenaminoandcarbonoxylgroupshasbeenexploited．Thenanob

4、iosensor,basedonthegoldnanoparticlesmodifiedwiththeorganicfunctionalgroupswithactivityincreased，exhibiteddistinctopticalpropertiesoflocalizedsurfaceplasmonresonance(LSPR)invisibleabsorptionregion．Themaximumadsorptionamountofc-mycmonoclonalantibodymoleculeonthemodifiedLSPRnanob

5、iosensorwiththenovelorganicfunctionalnanofilmcouldreach2．4pg／mL．TheLSPRbiosensorimmobilizedwithc-mycmonoclonalantibodycouldbeusedforrecognitionofoncogenebiomoleculessuchasMYCrecombinantprotein(P01)withalowlinearresponserangeof6．5x10一一1．3I．tg／mLandadetectionlimitof6．5x10一I-tg／m

6、L．Nosignificantinterferencesfrombovineserumalbumin(BSA)，swinehemoglobulin，bovinealbumin，transferrin，humanhemoglobulin，thrombin，lysozymecouldbeobserved．Incomparisonwithenzymelinkedimmunosorbentassay(ELISA)，theLSPRnanosensorpossessedarecoveryof92．3l％一109．23％fordeterminationofsam

7、plescontainingc-mycproteinwithnicereproducibility,showingitspromisingfeasibility．Moreover，thepresentednanobiosensorhasbeensuccessfullyappliedtodetectionofamountofc—mycproteinintumorcells，showingitspotentialapplicationIIinanticancerpredictiveandtherapeuticstrategies．TheLSPRbios

8、ensorimmobilizedwithc-mycrecombinantproteincouldbeusedforreco