邻苯二甲酸二正戊酯高效降解菌的筛选、降解特性及其降解途径研究

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1、万方数据ABSTRACTSCREENINGOFHIGHLYEFFICIENTDEGRADATIoNBACTERIAoFDI．N．AMYLPHTHALATEANDITSDEGRADATIoNCHARACTERISTICSABSTRACTDi—n·amylphthalate(DAP)isacommonenvironmentalpollutantandiswellknownforitsendocrinedisruptingactivities．Widelyuseoftheplasticsandpest

2、icidesinagriculturehaveresultedinDAPpollutioninsoilsandwater．Inthisstudy，aDi·n·amylphthalate(DAP)degradingbacterialwasisolatedfromactivatedsludge．ItcouldutilizeDAPassolecarbonandenergysourcesforgrowth．Accordingtoitsphenotypiccharacteristics，physic-bi

3、ochemicalcharacteristicsand16SrRNAgenesequenceanalysis，strainWasidentifiedascomamonastestosteroniandnamedasLS1．ThestrainLS1grewwellinLBliquidmedium．ARer8h，itenteredintothelogarithmicgrowthand30--40hitenteredthestablegrowthperiod．Theoptimumtemperature

4、andpHforthegrowthofstrainLS1Was30"CandpH=7．0-8．0，respectively．LessliquidvolumeWasbetterforthegrowthofthestrainLS1．MoredissolvedoxygeninthewaterWasbenefitforthegrowthofthebacteria．Thestraincouldnotutilizeorganiccarbonsourcesuchasfructose，xylose，sucros

5、e，maltoseorglucose；Fornitrogensources，LSlcouldhighlyutilizepeptoneandfollowedareammoniumnitrogenandnitrate-N．LS1couldbarelyutilizenitrite-Norurea．ThestrainLS1withsalttolerancecouldgrowwellintherangeof0-159／lNaCIconcentrationwhichhadoutstandingadvanta

6、gesinwastewatertreatmentapplications．ThestrainLS1couldcompletelydegrade200mg‘L‘1DAPin48hinMSMculture；whenculturedinthemedium，theOD600increasedduringthedegradationprocess，whichindicatedthatthestraincoulduseDAPasthesolecarbonsourceforgrowth．Theoptimumt

7、emperatureandpHforDAPdegradationbystrainLS1were30。CandpHrangefrom7．0t08．0，respectively，whichwasthesameasitsgrowthcharacteristics．Thelowerinitialconcentration，theeasierutilizationofDAPbythestrain．Generally，thestraincouldutilizeaIII万方数据邻苯二甲酸--iE戊酯高效降解菌

8、的筛选、降解特性及其降解途径研究highconcentrationofDAP．LS1couldutilizePhthalicacidbutnototherPAEsastIlesolecarbonsourceforgrowth．A1000bpDNAfragmentwasobtainedfromthestrainLS1byPCRamplifiedandclone．ThedegradationintermediatesofDAPwasidentifiedasphthalateacid(PA)．Them