农业废弃物对多环芳烃污染土壤的修复效果与微生物解析

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1、题目农业废弃物对多环芳烃污染土壤的修复效果与微生物解析学院资源与环境学院专业环境工程班级环工1003学生孙延琛学号20102122135指导教师韩雪梅二O—四年六月三日摘要向多环芳烃(PAHs)污染土壤中添加农业废弃物能够为土壤中的微生物提供营养物质，从而提高土壤中可以降解PAHs菌的数量，目前的研宄主要关注农业废弃物对微生物提供营养，有关农业废弃物对微生物降解PAHs的机制尚不清楚。本项目将灭菌后的牛粪、蘑菇培养基废料和小麦秸秆分别添加到PAHs污染土壤中，测定多环芳烃污染土壤中不同形态、不同组分PAHs含量

2、的动态变化，揭示农业废弃物对PAHs的作用；监测多环芳烃污染土壤中农业废弃物的组成和结构性质变化，探明农业废弃物作为营养源被微生物利用和降解的特征；分析未灭菌土壤中PAHs降解菌的丰度和菌群结构以及不同形态、不同环数PAHs的降解率，确定微生物对PAHs的降解作用。综合以上的研宄内容，阐明农业废弃物作为微生物营养源强化微生物降解PAHs的机制，本研宄对于丰富PAHs污染土壤的生物修复理论以及促进PAHs污染土壤的治理具有长远的意义。关键词：土壤；多环芳烃；微生物；农业废弃物ABSTRACTMicrobialde

3、gradationofpolycyclicaromatichydrocarbons(PAHs)canbeenhancedthroughincreasingthesolubilityofPAHsandprovidingthenutrientsformicroorganismswhenaddingtheAgriculturalwaste(AW)tosoilscontaminatedbyPAHs.TherecentstudiesfocusontheeffectofAWonthesolubilityanddegrada

4、tionofPAHs.However，themechanismofAWenhancingthemicrobialdegradationofPAHsisstillnotclearuptonow.Inthisstudy,thesterilizedAWfromcowmanure，sludge，culturemediumwasteofmushroomorwheatstrawisaddedtosoilscontaminatedbyPAHsrespectively.ThesolubilizationeffectsofAWo

5、ndifferentformsandcomponentsofPAHsarerevealedbymeasuringthedynamicchangesoftheircontentincontaminatedsoils.ThecharacteristicsofmicrobialutilizationanddegradationofAWwhichservesasnutrientsourceofmicroorganismsareindicatedbydetermingthecomposition，structureand

6、propertyofAWincontaminatedsoilsatdifferentculturetime.ThedegradationofdifferentformsandcomponentsofPAHsbysoilmicroorganismsisrevealedbyanalyzingtheabundanceandcommunitystructureofPAHs-degradingbacteriaaswellasdegradativeratesofdifferentformsandcomponentsofPA

7、Hsincontaminatedsoilsatdifferentculturetime.Afterthesyntheticanalysisoftheaboveresearchcontentsismade，themechanismofAWenhancingthemicrobialdegradationofPAHsthroughservingasthesolubilizerforPAHsandnutrientsourceformicroorganismswillbeelucidated.Thisworkisofim

8、portantsignificancetoenrichthetheoryofenhancedbioremediationandimprovethetreatmentofPAHs-contaminatedsoils.Keywords：Soil；PAHs；microorganism；Agriculturalwaste目录J商要-IABSTRACTTT1W-1-1.1多环芳烃概述-1-1.1