吗啡预处理对阿霉素所致的慢性充血性心力衰竭大鼠心肌细胞缺氧再给氧损伤的保护作用

《吗啡预处理对阿霉素所致的慢性充血性心力衰竭大鼠心肌细胞缺氧再给氧损伤的保护作用》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、安徽医科大学硕士学位论文种阻断剂分别在预处理前10min分别加入非选择性阿片受体阻断剂纳洛酮（10μmol/L）和δ-阿片受体阻断剂钠曲吲哚（1μmol/L），分别检测缺氧前后和再给氧前后培养液中LDH水平和CK的活性，采用胎盘蓝染色和MTT检测处理后的各组心肌细胞活力，Hoechst33234染色检测凋亡率。结果与CON组相比，H/R组心肌细胞活力明显降低，凋亡细胞数增加，而LDH和CK活性明显升高（P<0.05）。HPC较H/R组未见明显改善，而MPC组呈剂量依赖性改善细胞H/R后的损伤，且1

2、μmol/L可能为最佳剂量，增加细胞存活率47.4%，降低LDH释放38.1%。纳洛酮可完全阻断MPC的心肌保护作用，而钠曲吲哚部分阻断该作用。结论缺氧预处理对对阿霉素所致的慢性充血性心力衰竭大鼠心肌细胞缺氧再给氧损伤无保护作用，吗啡预处理有保护作用，且该作用可能是通过激活阿片受体介导的，主要为δ-阿片受体。关键词心力衰竭吗啡预处理缺氧再给氧心肌保护7安徽医科大学硕士学位论文Cardioprotectioneffectofmorphinepreconditioningoncardiomyocyte

3、shypoxia-reoxygenationinjuryofAdriamycin-inducedadultchroniccongestiveheartfailureABSTRACTObjective:Heartfailureisaseriousthreattothehealthofthepatientasacommonclinicalcriticallyillpatient,whichisthefinalperformanceofavarietyofcardiovasculardiseases.W

4、etestedwhethermorphinepreconditioningretainsitscardioprotectioninchroniccongestiveheartfailureratinducedbyAdriamycininvitro,andtofindthepossiblemechanism.AndAndramycinheartdamagehasbeenpaidmoreandmoreattentionforthereasonthatthedrugs’obviouseffectonhu

5、mantumorinclinical.Ontheotherhand,it’scommonthatheartischemia-reperfusioninjuryownsnormalhighincident,whichhasbeenprotectedbytheclassicischemiapreconditioning.Methods:ChroniccongestiveheartfailurewasinducedbytailveininjectionofAdriamycin(2mg/kgweeklyf

6、or6weeks)inmale220-250gmailSprauge-Dawleyrats.Isolationofcardiomyocytesusing0.05%typeIIcollagenasewasperformedinthesecondweekafterthelastinjectionofadriamycin,andtreatedwithhypoxiapreconditioning(HPC)ormorphinepreconditioning(MPC,0.03,0.1,0.3,1,3μmol/

7、L)after48hnormoxicculture.MPCwasinducedbythreecyclesof5-minperfusionofmorphineinterspersedwith5-minofdrug-free.NaloxoneandNaltrindolewereaddedat10minbeforehypoxia-reoxygenationfor8安徽医科大学硕士学位论文observingthemechanism(s)inthisprocess.Andcardiomyocyteswere

8、dividedintoninegroups:controlgroup(CON),hypoxiapreconditioninggroup(HPC),morphinepreconditioninggroup(MPC,1μmol/L),HPCandNaloxone(HPC+Nal),HPCandNaloxone(HPC+Nat),MPCandNaloxone(MPC+Nal),MPCandNaloxone(MPC+Nat)andNaloxoneandNaloxonegroupasself