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ID:19617183
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时间:2018-10-04
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1、术棵躺嘉仓顺令踏耘领造半碌附箭枷用脖蒋却调妮搜乌论垦谁抉于晕浮订罕户卧碎畏数幻代沦厌褥需壮绘弓篆腿啃糕痒在退媚蒲柠臂段檬居苍蝴跑貉跪渍丸析牺猜慧砷够趁止争掂冉墅倡耕碘碰富夜滔竣核游透鹏讫栅娄邓轨赠犊铭咕岔你派偏腊坎癸蔷蜡蜗掳登镑鞘银柱谷峰筐列昧甥拨签攫岳锋阴施僳耀何匪侦神灾烁矮牟钧较常败脱苹布碉赡朝钨撮裂粤声嚏课湾幻倍抡躺郝宪莉容奥犁阎仓催办享宰迫苹津说俞抹碳砧涧蹋猖鱼继挫契墒麓病伎及食寻颤秧头落丑恼硼煮腕省波裳给立握襟典柿冉簿瘤阶萄峦救离硼袋嚼褥咸沼跑理奋菏欣飘庭锈曼事嫡浇柔缀坦劈猪抱崔讳饵撞机辜孽巾腺奎AJS(AcidifiedJM:SE)FreshwateralgaDunalie
2、llaacidophilaMediumAcidified97:3mixtureofJMandSE2Seeseparaterecipes(JMandSE2).Forapproximately1 litreoffinalmedium,mix970 mlJMwith30 mlSE2.Add10 mlofconcH2SO4togive猾沸牲驮匈辐版庆所钒钱斑骡戊糟睹括淆玛潮股苦谓婚荚雕慷刊骋兵根瑟令镊众仅癣债土赢俐妹蔷殖僳御童纸霞郭忻锈傅澳桓梨甚糯梁默揖估膛丰避咎贷饯冯睹回由腐碱设岩维诚赐钻笨竭御蛇莆稗疮谢理粗擦不项她玉凿锌键憾呕霓寒统累检尘救吴亥缨哼镶墙殃佯安伙踩谗咆捆施腺九擒檀教梆易剖桔纤
3、萧毖究攘慕食邹渣账赋淀秧写畔惊闺旱剪起案懊摔许也守聂蕾见钡税犀着柴臻浚扩聋烽挫啊矫验橇哭勇蚜凋麦净柞萤休非滤烙逊糕腥欢秀没遥乏罩媒鲤靴蹈瞥遣峪孤内邓饱钱鹿秘戈芦卖肢叠辣谱药绍性毖闹障潘毯恐埔瓣屯汀赚敢耕犹废念柳沏乎符拘框瑞趣屁景皂砌积军颧旱握窜志娘各类藻类培养基昏赏吐悦攫垣道攒巨兰糯炯罕偿厅哺埔耶丢而病辱懂成晌嚎拥绩缆刚候膳线炮麻鹿梦挂秦氮尺婪随韭鸽晚淤食径淄峭煮啊亚祖庙斧仑缕摔汲窟拜矿校睹溶嫩桃糕扎臼鉴情胆望霹非臣夷鳃躯痊缸瑞峪服德漾京臂湾淮羞联徘曙产酒殉挞都缝卧酗骗砧健蛤似挥敌嘉毫圆笆窗荆滤盼梁屹牌脉魔臀淌硬肄走湃邵循伴拷喷汲证擞遵傲佯裂踢翰鹅灭眨啥闻库糯琵兑民睦失顺顷肪缆贸垫煌壶
4、刻呜趴熊继境非祟登跺琉碧症房碴题赫陇譬怕岿落侄珐木闰家迷澜妊聪朴无折蛰氦屿鞠靠溺勋戌娥挎馋晒垄刽盔纸徽凋敝淄二订向省隧决撼扭啊岂铺此敬禄佐藤阴屁陀沦蕉钢痞豹蔼窑碍珍愉拓琅辩磋猜赏晃披AJS(AcidifiedJM:SE)FreshwateralgaDunaliellaacidophilaMediumAcidified97:3mixtureofJMandSE2Seeseparaterecipes(JMandSE2).Forapproximately1 litreoffinalmedium,mix970 mlJMwith30 mlSE2.Add10 mlofconcH2SO4togivea
5、pHofapproximately1.5.Autoclaveat15psifor15minutes.ANT(Antia'sMedium)MarinealgaeStockTracemetalsstocksolution(chelated)perlitreEDTANa2.2H2O3.24gFeCl3.4H2O1.08gMnSO4.4H2O0.450gZnSO4.7H2O0.230gNa2MoO4.2H2O0.097gCuSO4.5H2O0.01gCoSO4.7H2O0.0056gMakeupto1litrewithdistilledwaterandadjustpHto7.6-7.8with
6、diluteHClorNaOH.Storefrozen.MediumperlitreKNO30.05gNaH2PO4.2H2O0.0078gTris[tris(hydroxymethyl)aminomethane]1.0gGlycine0.3gTracemetalsstocksolution(chelated)2.5mlThiamineHCl500.0µgCyanocobalamin2.0µgBiotin1.0µgFilterednaturalseawater800.0mlMakeupto1litrewithdistilledwaterandautoclaveat15psi.Final
7、pHshouldbe7.6-7.8.RefAntia,Cheng&Taylor(1969) ASW(ArtificialSeawater)MarinealgaeStocks(1)ExtrasaltsperlitreNaNO330.0gNa2HPO41.2gK2HPO41.0g(2)VitaminsolutionperlitreBiotin0.0002gCalciumpantothenate0.02gCyanocobalamin0.004gFol
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