女性输卵管堵塞的治疗女人输卵管堵课件

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1、OverviewofAffymetrixGeneChipTechnologyGeneChipTechnologyAffymetrixIncMiniaturized,highdensityarraysExpressionarrays1,300,000DNAoligos1-cmby1-cmDNAmappingarray7,000,000DNAoligo1.3by1.3cmManufacturingProcessSolid-phasechemicalsynthesisandPhotolithographicfabricationt

2、echniquesemployedinsemiconductorindustryPhotolithographicSynthesisManufacturingProcessProbearraysaremanufacturedbylight-directedchemicalsynthesisprocesswhichenablesthesynthesisofhundredsofthousandsofdiscretecompoundsinpreciselocationsLampMaskChipAffymetrixWaferandC

3、hipFormat1.0cm20-50µm20-50µmoneoligonucleotidesequenceper“pixel”49-400chips/waferupto~1.3millionfeatures/chipSelectionofExpressionProbesSetofoligostobesynthesizedisdefinedfromsensesequenceofknowngenesandEST’sProbesSequencePerfectMatchMismatchChip5’3’Computeralgori

4、thmsareusedtodesignphotolithographicmasksforuseinmanufacturingEachgeneisrepresentedontheprobearraybymultipleprobepairsEachprobepairconsistsofaperfectmatchandamismatcholigonucleotideOverview:CreatingTargetsReverseTranscriptaseinvitrotranscriptionmRNAcDNAFragmentatio

5、nofcRNAcRNAGeneChipHybridizationThisiswhatwearedoinginthisclassmRNAcRNAFragmentationofbiotinylatedcRNAFragmentation-MetalmediatedalkalineinducedhydrolysisHybridizationandStainingArrayFragmentedcRNATargetRNA:DNAHybridizedArrayStreptavidinphycoerythrin[Fluorescentdye

6、]InstrumentationAffymetrixGeneChipSystem3000-7GScanner450FluidicStation640HybridizationOvenCurrentlyAvailableGeneChipsB.subtilisBarleyGenomeArrayBovineGenomeArrayC.elegansGenomeArrayCanineGenomeArrayChickenGenomeArrayDrosophilaGenomeArraysE.coliGenomeArraysHumanGen

7、omeArraysMaizeGenomeArrayMouseGenomeArraysP.aeruginosaGenomeArrayPlasmodiumGenomeArrayPorcineGenomeArrayRatGenomeArraysRiceGenomeArraySoybeanGenomeArraySugarCaneGenomeArrayVitisvinifera(Grape)ArrayWheatGenomeArrayXenopuslaevisGenomeArrayYeastGenomeArraysZebrafishGe

8、nomeArrayArabidopsisGenomeArraysExpressionArraysDNAArrays10KDNAmapping100KDNAmapping500KDNAmappingExonArrayResequencingSNParraysCustomGeneChipsAf