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时间:2018-09-15
《antioxidation+activities+of+low-molecular-weight+gelatin+hydrolysate+isolated+from+the+sea+cucumber+stichop+us+japo+nic+us》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、J-OclNlnUniv.China(OceanicandCoastalSeaResearch)DOI10.1007/s11802-010—0094—9ISSN1672—5182,20109(1):94.98http://www.ouc,edu.cn/xbywb/E.mail:xbywb@ouc.edu.cn~tioxidationActivitiesofLow-Molecular-WeightGelatinIsolatedfromtheSeaCucumberWANGJingfeng,WANGYuming,TANGQingjuan,WANGYi,CHAN
2、GYaoguang,ZHAOOin,andXUEChanghu'CollegeofFoodScienceandEngineering,OceanUniversityofChina,Qingdao266003,P.R.China.(ReceivedMay21,2009;revisedSeptember18,2009;acceptedNovember9,2009)@OceanUniversityof’China.SciencePressandSpringer-VedagBerlinHeidelberg2010AbstractGelatinextractedf
3、romthebodywalloftheseacucumber(Stichopusjaponicus)washydrolyzedwithflavourzyme.Low-molecular-weightgelatinhydrolysate(LMW-GH)of700-1700Dawasproducedusingallultrafiltrationmembranebioreae-torsystem.ChemiluminescenceanalysisrevealedthatLMW—GHscavengeshighfreeradicalsinaconcentratio
4、n-dependentmail—ner;IC5ovalueforsupemxideandhydroxylradicalswas442and285ggmL~,respectively.LMW-GHexhibitedexcellentinhibi—torycharacteristicsagainstmelaninsynthesisandtyrosinaseactivityinB16cells.Furthermore,LMW—GHnotablyinc~edin-traeellularglutathione(GSH),whichinturnsuppressedm
5、elanogenesis.LMW-GHperformsantioxidationactivity,holdingthepotemialofbeingtlsedas"avaluableingredientinfunctionfoods.cosmeticsandpharmaceuticalsornutriceuticals.Keywordsseacucumber;low-molecular-weightgelatinhydrolysate;antioxidationactivity;antimelanogenesis;tyrosinase;re出lcedgl
6、utathionepeptidesderivedfromaquaticanimalsonmelanogenesishavenotbeenstudied.1lntroductionSeacucumberisatraditionaltonicfoodinChmaandFishproteinhydrolysateshavenoticeableantioxidationotherAsiancountriesduetoitsanti·-tumor,immunoregu—·properties,which:mayserveasscavengersofreactive
7、latory,anti-atheroscleroticandanti-agingproperties(Hi—oxygenspecies(ROS)(Kimeta1.,2001;Mendiseta1.,rataeta1.,2095;Wangeta1.,2007).Collagenaccounts2005:Yangeta1.,2008).Gelatinpeptidesarecomposedforapproximately70%ofthetotalproteinofthebodyofmain~hydrophobicaminoacidswhichcouldincr
8、easewallofseacucumber(Saitoeta1.,2002),w
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