antioxidation+activities+of+low-molecular-weight+gelatin+hydrolysate+isolated+from+the+sea+cucumber+stichop+us+japo+nic+us

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1、J-OclNlnUniv．China(OceanicandCoastalSeaResearch)DOI10．1007／s11802-010—0094—9ISSN1672—5182，20109(1)：94．98http：／／www．ouc,edu．cn／xbywb／E．mail：xbywb@ouc．edu．cn~tioxidationActivitiesofLow-Molecular-WeightGelatinIsolatedfromtheSeaCucumberWANGJingfeng，WANGYuming，TANGQingjuan，WANGYi，CHAN

2、GYaoguang，ZHAOOin，andXUEChanghu'CollegeofFoodScienceandEngineering,OceanUniversityofChina,Qingdao266003，P．R．China．(ReceivedMay21，2009；revisedSeptember18，2009；acceptedNovember9，2009)@OceanUniversityof’China．SciencePressandSpringer-VedagBerlinHeidelberg2010AbstractGelatinextractedf

3、romthebodywalloftheseacucumber(Stichopusjaponicus)washydrolyzedwithflavourzyme．Low-molecular-weightgelatinhydrolysate(LMW-GH)of700-1700Dawasproducedusingallultrafiltrationmembranebioreae-torsystem．ChemiluminescenceanalysisrevealedthatLMW—GHscavengeshighfreeradicalsinaconcentratio

4、n-dependentmail—ner；IC5ovalueforsupemxideandhydroxylradicalswas442and285ggmL～，respectively．LMW-GHexhibitedexcellentinhibi—torycharacteristicsagainstmelaninsynthesisandtyrosinaseactivityinB16cells．Furthermore，LMW—GHnotablyinc~edin-traeellularglutathione(GSH)，whichinturnsuppressedm

5、elanogenesis．LMW-GHperformsantioxidationactivity,holdingthepotemialofbeingtlsedas"avaluableingredientinfunctionfoods．cosmeticsandpharmaceuticalsornutriceuticals．Keywordsseacucumber；low-molecular-weightgelatinhydrolysate；antioxidationactivity；antimelanogenesis；tyrosinase；re出lcedgl

6、utathionepeptidesderivedfromaquaticanimalsonmelanogenesishavenotbeenstudied．1lntroductionSeacucumberisatraditionaltonicfoodinChmaandFishproteinhydrolysateshavenoticeableantioxidationotherAsiancountriesduetoitsanti·-tumor,immunoregu—·properties，which：mayserveasscavengersofreactive

7、latory,anti-atheroscleroticandanti-agingproperties(Hi—oxygenspecies(ROS)(Kimeta1．，2001；Mendiseta1．，rataeta1．，2095；Wangeta1．，2007)．Collagenaccounts2005：Yangeta1．，2008)．Gelatinpeptidesarecomposedforapproximately70％ofthetotalproteinofthebodyofmain~hydrophobicaminoacidswhichcouldincr

8、easewallofseacucumber(Saitoeta1．，2002)，w