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1、中国农业科学2006,39(11):2243-2249ScientiaAgriculturaSinica小麦抗源Holdfast和Flinor抗条锈病主效、微效基因的遗传分析1,21,21仝淑玫,蔺瑞明,何月秋,徐世昌1(中国农业科学院植物保护研究所／植物病虫害生物学国家重点实验室，北京100094;2云南农业大学农作物多样性病虫害控制教育部重点实验室，昆明650201)摘要：【目的】Holdfast、Flinor是国际上已经报道在生产上具有持久抗条锈性表现的小麦品种，在成株期对中国条中29、30、31号小种表现出免疫或近免疫的抗性
2、水平。【方法】本文采用常规杂交分析和潜育期变温处理方法，分别在常温（昼18℃/夜10℃）和高温（昼24℃/夜15℃）两种温域下，对其主效和微效抗条锈病基因进行遗传分析。【结果】品种Flinor中至少含有1显1隐2对主效和2对温敏微效抗条锈基因，Holdfast中至少含有2对显性主效和2对温敏微效抗条锈基因。主效基因均互补控制其抗条锈性,微效基因呈隐性，具累加效应，受高温诱导表达，控制中度抗性水平。【结论】Flinor和Holdfast均含有全生育期主效抗条锈病基因和对高温敏感的微效抗条锈基因，建议在抗病育种中加以有效利用，并提供了温
3、敏微效抗条锈基因的快捷检测方法。关键词：小麦；抗条锈；主效基因；微效基因；遗传分析GeneticAnalysisofMajorandMinorGene(s)ResistanttoStripeRustinResourceWheatCultivarsHoldfastandFlinor1,2121TONGShu-mei,LINRui-ming,HEYue-qiu,XUShi-chang12(InstituteofPlantProtection,ChineseAcademyofAgriculturalSciences,Beijing1000
4、94;KeyLaboratoryofCropsDiversityforPlantDiseasesandPestsControl,MinistryofEducation,YunnanAgriculturalUniversity,Kunming650201)Abstract:【Objective】Twowheat(Triticumaestivum)cultivars,HoldfastandFlinor,werereportedtobedurablyresistanttostriperust(Pucciniastriiformis).Th
5、eywereimmuneornearlyimmunetotheracesCY29,CY30andCY31ofPucciniastriiformisatadultstage.【Method】Inthiswork,weanalyzedtheirinheritanceofmajorandminorresistance(R)genesthroughmakingcross.Theresistanceanalysiswasdevelopedattwotemperaturemodels,thenormaltemperaturemodel:18℃d
6、uringthedayand10℃atnight,andthehightemperaturemodel:24℃duringthedayand15℃atnight.【Result】FlinorhasatleasttwomajorRgenes,onedominantandanotherrecessive,plustwotemperature-sensitiveminorRgenes.HoldfasthastwodominantmajorRgenesandtwotemperature-sensitiveminorRgenes.Themaj
7、orRgenesarecomplementarily,whereastheminorRgenesarerecessive.Hightemperatureinducedanddominatedmoderateresistancetostriperustinanadditivefashion.【Conclusion】BothFlinorandHoldfasthavemajorRgenealongthewholegrowthdurationandtemperature-sensitiveminorRgene.Thestudysuggest
8、sthattwocultivarsbeutilizedinwheatresistancebreeding.Inaddition,arapididentificationmethodwasdevelopedforatemperature
