SNT1155-2002植物检疫玉米霜霉病菌检疫鉴定方法.pdf

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1、中华人民共和国出入境检验检疫行业标准SNIT1155-2002植物检疫玉米霜霉病菌检疫鉴定方法Plantquarantine-MethodsforquarantineandidentificationofPeronosclerospora(lto)C.G.Shaw2002一11一25发布2003一05一01实施中华人民共和国发布国家质量监督检验检疫总局SN/T1155-2002目次前言···。···················，······‘·····，···。··················

2、···，·········，，··················，···············，····一I1范围····································，···········，······························································⋯⋯12原理···································································，·········

3、······················，··，·····一13仪器和用具······································································，···························⋯⋯14试剂和材料····································································································⋯⋯15现场检

4、疫与取样‘。。·····，·····‘·················，··········，·，·······，，，，，，··，，，，·，····，，，，···⋯⋯26实验室检验··············，·······················，··························································，···⋯⋯37结果评定·····························，·······················

5、·······，··········，··⋯⋯‘············，·⋯⋯58样品保存·········。··············································，················································⋯⋯5附录A(资料性附录)玉米霜霉病菌寄主范围·························································⋯⋯6附录B<资料性附录)DNA提取、扩增与

6、电泳，···························，························，··⋯⋯7附录C(规范性附录)PCR扩增引物·................................................................“一’二’“9附录D(资料性附录)玉米霜霉病菌卵抱子、分生抱子、分生抱子梗图···························⋯⋯10SN/T1155-2002.几J..叫.-月Ili昌本标准的附录C为规范性附录，附

7、录A、附录B、附录D为资料性附录。本标准由国家认证认可监督管理委员会提出并归口。本标准起草单位:中华人民共和国福建出人境检验检疫局。本标准主要起草人:李德福、郭琼霞、陈艳、张广志、张晓燕。本标准系首次发布的检验检疫行业标准。SN/T1155-2002植物检疫玉米霜霉病菌检疫鉴定方法范围本标准规定了植物检疫中玉米霜霉病菌检疫和鉴定方法。本标准适用于玉米、甘蔗、高粱及其他寄主中玉米霜霉病菌的检疫和鉴定。2原理玉米霜霉病主要由高粱霜霉病菌(Peronosclerosporasorghi)、甘蔗霜霉病菌(P.

8、sacchari)、菲律宾霜霉病菌(P.philippinen.sis)和爪哇霜霉病菌(P.maydis)引起，该类病菌(以下简称玉米霜霉病菌Peronosclerosporaspp.)属鞭毛菌亚门(Mastigomycotina),梦p菌纲(Oomycetes),霜霉目(Peronospora-les),霜霉科(Peronosporaceae)、霜指霉属(Peronosclerospora)。玉米霜霉病菌除危害玉米外，还侵染其他植物(参见附录A)。一般