结合gc-ms的分子蒸馏富集柚皮精油抗氧化成分的研究

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1、结合GC-MS的分子蒸馏富集柚皮精油抗氧化成分的研究王芳1,2岳朝敏1AndreeaDavid1林洁茹1何宇城2邓刚1（浙江师范大学化学与生命科学学院1，金华321004）（浙江师范大学行知学院2，金华321004）摘要分子蒸馏技术富集柚皮精油的抗氧化成分，经两次分子蒸馏后，得到两种重馏分(1RH、2RH)、两种中间馏分(1DM、2DM)和一种轻馏分(1DL)。分子蒸馏过程中，采用气相色谱-质谱联用技术(GC-MS)实时分析了各馏分的化学组成，确定了33种化合物。通过测定各馏分清除DPPH.和ABTS+.的能力得知，香叶醇和β-石竹烯是主要的抗氧化物质，被富集到重馏分(

2、2RH)中，浓缩比分别为3.7和4.3，同时D-柠檬烯在轻馏分(1DL)中得到明显富集，浓缩比达4.9。抗氧化实验表明重馏分(2RH)的抗氧化能力较原油得到了明显提升，清除DPPH.和ABTS+.的能力分别比原油提高了60%和75%。分子蒸馏技术不仅用于分离脂肪和脂肪酸，而且在植物精油精制及提高附加值方向也具有广阔的应用前景。关键词分子蒸馏抗氧化活性精油中文分类号：TS225.1文献标识码：AEnrichmentofAntioxidantComponentsofPomelo(CitrusGrandis(L.)Osbeck)EssentialoilusingMolecul

3、arDistillationCombinedwithGC-MSDeterminationWangFang1,2YueChaomin1AndreeaDavid1LinJieru1HeYucheng2DengGang1(CollegeofChemistryandLifeSciences,ZhejiangNormalUniversity1,Jinhua321004)(XingzhiCollegeZhejiangNormalUniversity2,Jinhua321004)AbstractMoleculardistillationwasusedtofractionatethee

4、ssentialoilofpomeloaimingtoenrichantioxidantcomponentsinthispaper.Aftertwo-stagemoleculardistillation(TMD)attheoptimaloperationconditions,thefractionsincludingtwoheavyresidue(1RH,2RH),twomiddledistillate(1DM,2DM)andlightdistillate(1DL)canbecollected.Gaschromatography-massspectrometry(GC-

5、MS)wasemployedtoanalyzethechemicalcompositionsforeachfractionduringthemoleculardistillation.IncombinationwithantioxidantactivityevaluationincludingDPPHandABTSassays,geraniolandβ-caryophyllenecanbeidentifiedasthetwomajorantioxidantcomponentswithin33compoundsofthecrudeoil(Co).Finalresultss

6、howthatthosemajorantioxidantcomponentscanbeenrichedinthefraction2RHwithhighconcentrationratioapproaching3.7and4.3,respectively,whereasD-limonenecanbeapttothelightfraction1Dwithconcentrationratiocloseto4.9.Moreover,free-radicalscavengingactivitywasdeterminedforallfractionsandwashighestin2

7、RH,increasingby60%inDPPHand75%inABTSgreatlyhigherthanpomelocrudeoil.Inconclusion,notonlycanmoleculardistillationbeappliedforseparationoffatorfattyacid,butitisapotentialandpromisingtechniqueforrefiningtheplantessentialoilsandforimprovingthepropertiesoftheirproducts.Keyword