peprotech公司 elisa操作指南视频(视屏地址、字幕及翻译)

《peprotech公司 elisa操作指南视频(视屏地址、字幕及翻译)》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、学习啦-PeproTechELISA操作指南视频已上传!为新手能够更好的熟悉和掌握PeproTech的ELISA试剂盒及其技术，PeproTech专门制作了"ELISA操作指南视频"，供各位老师和同学观摩！视频网址为：http://v.youku.com/v_show/id_XMzQ3Mzk3NTg4.html或http://www.tudou.com/programs/view/tzU8m93o0tI/，欢迎大家的批评和指正！该视频为英文讲解，为便于大家理解，请看下面的英文原文及翻译：Thefo

2、llowingvideotutorialdescribedhowtorunasandwichELISA,alsoknownasenzyme-linkedimmunosorbentassay,usingageneralPeproTechprotocol.接下来的实验指导讲述PeproTech夹心法ELISA，即酶联免疫吸附试验的通用步骤Thecapture,standard,anddetectionaresuppliedasstablelyophilizedproducts,andshouldbes

3、toredat-20℃untilreadyforuse.捕获抗体、标准品和检测抗体均为冻干粉，使用前应保存于-20℃ReconstitutedCapture,Standard,andDetectioncomponentsareonlyguaranteedtobestableforupto2weekswhenstoredat4℃.捕获抗体、标准品和检测抗体重悬后，在4℃时最长保存2周。IfyouhavereconstitutedtheEDK,andplanonusingitforadurationg

4、reaterthantwoweeks,aliquotandstoreat-20℃forupto6months.如果您已重悬了EDK的各组份，并准备在2周之后使用，请将重悬的组分分装并冻存于-20℃，最长可保存6个月。IncontrasttotheotherthreeEDKcomponents,theAvidin-HRPvialcomesreadytouse与EDK的其它三个组分不同，Avidin-HRP为即用型Inordertoavoidharmfulrepeatedfreeze/thawcycl

5、es,orlong-termstorageat4℃whichmeanadvisoryfunctionality.AliquottheAvidin-HRPintoten6uLvialsuponreceiptandstoredat-20℃arestableforup2yearsfromthedateofreceipt.目的是避免反复冻融或长期4℃保存对该组分的功能损害。收到Avidin-HRP后，立即将其分装为6ul/管，共10管，冻存于-20℃，最长可保存2年Phase1:第1阶段：Coatingt

6、heplatewithcaptureantibody捕获抗体包板Centrifugethevialbrieflytobringthecaptureantibodytothebottom将捕获抗体稍作离心，使抗体集中于管底Reconstitutethecapturebodyinsterilewatertotheconcentrationspecifiedonthedatasheet.Andallowthevialtobereconstitutedforminimum10minutes.用无菌水将捕获

7、抗体重悬至说明书上要求的浓度，静置10分钟以使抗体完全溶解Centrifugethereconstitutedvialfor3minutesatmaximumspeed重悬的抗体以最高速度离心3分钟Dilutethecaptureantibodyin1×PBStotheconcentrationspecifiedonthedatasheet.用1xPBS稀释捕获抗体至说明书上要求的浓度Gentlymixorvortexthevial,trytoensuretheairbubblesdon’tmix

8、intothesolution,轻轻颠倒或振荡混匀，一定不要产生气泡Immediatelyadd100ulofthecaptureantibodysolutionintotheELISAplatewells.立即在每个ELISA板孔中加入100uL捕获抗体Pressfirmlytosealtheplate,takecarenottoletthereagentsplashonthefilm将封板膜用力压盖在ELISA板上，注意不要使抗体滴溅在封板膜上Incubatedtheplate