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1、[键入文字]动物生理学实验报告生理学实验报告实验内容:一、蛙的坐骨神经-腓肠肌标本的制备二、骨骼肌收缩的实验课程名称:动物生理学实验指导老师:实验人:院系专业:学号:2010年10月20日methodofbasicoperation;includingCT,andMRIvariouscontrast,andImage-guidedbiopsy,ultrasound,etc.(11)teaching,scientificresearchandtraininginclinicalteaching,writtenwi
2、thinthreeyearswithsomelevelofrevieworbookreportsofnotlessthan1.Four,toreadreferencebooks,Journalofinternalmedicine(medicalcollegeplanningmaterials)andofthemedicalimaging(medicalcollegeplanningmaterials);thepracticalscience;theHarrisonScience(inEnglish),Chine
3、seJournalofinternalmedicineseries.SurgicalsurgicalspecialistreferstotherulescompletethemedicalundergraduateorgraduateeducationFoundation,acceptedandadoptedsurgicalspecialisttraining,surgicaldiagnosis,treatment,preventionandfollow-upofcommondiseases,surgicald
4、iagnosisandtreatmentofrareorincurablediseases,acuteandcriticalillnessinemergencyandrescuehavepreliminaryknowledgeandexperience.Surgicalspecialisttrainingfor3years.Asurgicalspecialist,traininggoalsthroughtraining,traineestoachievesurgicalspecialistlevel,withm
5、oreskilledsurgicalskills,canguidetheteachingof第11页共11页[键入文字]动物生理学实验报告实验内容一蛙的坐骨神经-腓肠肌标本的制备(4-1)【实验目的】1、学习蛙类动物单毁髓与双毁髓的方法。2、学习并掌握蛙类坐骨神经-腓肠肌标本的制备方法。【实验原理】蛙类的一些基本生命活动和生理功能与恒温动物相似,而其离体组织所需的生活条件比较简单,易于控制和掌握。因此在实验中常用蟾蜍或青蛙的坐骨神经-腓肠肌标本来观察兴奋与兴奋性、刺激与肌肉收缩等基本生理现象和过程。制备坐骨神经
6、-腓肠肌标本室生理学实验中必须掌握的一项基本技能。【实验器材】常用手术器械(包括粗剪刀、手术剪、手术镊、眼科剪、眼科镊、金属探针、玻璃分针)、固定针、锌铜弓、蜡盘、培养皿、废物缸、棉线、纱布、滴管、任氏液。【实验对象】蛙【实验步骤】1、毁脑和脊髓取青蛙一只,用纱布包裹青蛙的四肢和躯干,露出头部。左手握住青蛙,并用食指按压头部前端,拇指按压背部使头部前俯;右手找到青蛙枕骨大孔所在位置。将探针由凹陷处垂直刺入,刺破皮肤即进入枕骨大孔,这时将探针由枕骨大孔转向头方,向前探入颅腔内,然后向各个方向搅动探针,以捣毁脑组织
7、。如探针确实在颅腔内,可感觉出针在四面皆壁的腔内。脑组织捣毁后,将methodofbasicoperation;includingCT,andMRIvariouscontrast,andImage-guidedbiopsy,ultrasound,etc.(11)teaching,scientificresearchandtraininginclinicalteaching,writtenwithinthreeyearswithsomelevelofrevieworbookreportsofnotlessthan
8、1.Four,toreadreferencebooks,Journalofinternalmedicine(medicalcollegeplanningmaterials)andofthemedicalimaging(medicalcollegeplanningmaterials);thepracticalscience;theHarrisonScience(inEnglish),Ch