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1、三草安前汤对慢性非细菌性前列腺炎模型大鼠IL-1β、IL-6及TNFRⅡ表达的影响作者：周青,熊伟,张国民,方圆,肖红玉,龚秀英【摘要】目的观察三草安前汤对慢性非细菌性前列腺炎模型大鼠炎症因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)及肿瘤坏死因子受体Ⅱ(TNFRⅡ)基因和蛋白表达的影响,阐明中药复方治疗慢性非细菌性前列腺炎的炎症因子蛋白靶点。方法将72只健康雄性Wistar大鼠随机分为6组,每组12只,除正常组外,其余5组应用大鼠前列腺蛋白提纯液辅以免疫佐剂制备实验性慢性非细菌性前列腺炎大鼠
2、模型,正常组、模型组予生理盐水,西药组予消炎痛,三草安前汤大、中、小剂量组分别予不同剂量的三草安前汤连续灌胃。30d后处死,采用免疫组织化学、荧光定量RT-PCR等方法检测炎症因子mRNA及蛋白表达。结果三草安前汤可显著降低慢性非细菌性前列腺炎模型大鼠炎症因子IL-1β、IL-6的mRNA及蛋白表达水平(P＜0.01),且大、中剂量组作用优于小剂量组(P＜0.05),大、中剂量组与正常组比较差异无统计学意义(P＞0.05)；三草安前汤各剂量组TNFRⅡ表达降低,未随剂量增加出现明显变化(P＞0.05)。结论三
3、草安前汤对慢性非细菌性前列腺炎模型大鼠异常的免疫功能有调节作用,降低炎症因子表达是其治疗慢性前列腺炎的重要机理。【关键词】12三草安前汤；慢性非细菌性前列腺炎；炎症因子；动物模型 Abstract：ObjectiveToobservetheeffectsofSancaoanqiandecoctiononexpressionofcytokineIL-1β,IL-6andTNFRⅡinexperimentalautoimmuneprostatitisrats,andexplorethetargetprotein
4、ofcytokineofChineseherbalcompoundintreatingchronicnonbacterialprostatitis.MethodsSeventy-twomaleWistarratsweredividedrandomlyintosixgroupsand12ratsineachgroup.AllgroupsweremadeexperimentalautoimmuneprostatitisratmodelbyinjectingSCpurifiedprostateproteinwith
5、FCAexceptnormalgroup.Thenormalandmodelgroupweregivensaline,westernmedicinegroupwasgivenindomethacin,large,mediumandsmalldosesdecoctiongroupsweretreatedwithlarge,mediumandsmalldosesofSancaoanqiandecoction.Allratsweresacrificedafter30days.ThemRNAandproteinexp
6、ressionofcytokinesweretestedbymethodsofimmunohistochemistryandfluorescentquantitationRT-PCR.ResultsSancaoanqiandecoctioncouldreducethemRNAandproteinexpressionofIL-1β,IL-6inexperimentalautoimmuneprostatitisrats.Therewerenosignificantdifferencebetweenlargeand
7、mediumdosesdecoctiongroup,buthadabetteractivitythansmalldose.TheexpressionofTNFRⅡwerereducedinall12dosesgroupsandnodifferenceamongthreedosesofSancaoanqiandecoction.ConclusionSancaoanqiandecoctioncanregulatetheimmunefunctioninchronicprostatitisratmodel.Down-
8、regulationofcytokinesmaybeoneofimportantmechanismsintreatingchronicprostatitis.Keywords：Sancaoanqiandecoction；chronicautoimmuneprostatitis；cytokine；animalmodel慢性非细菌性前列腺炎(CAP)是男科临床常见的难治性疾病之一,炎症因子在CAP的发病
