h2s对兔离体心脏心率的影响

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1、H2S对兔离体心脏心率的影响作者：陈立锋徐涛范凌赵兰平陈少峰温晓竞张晓云马建伟尹万斌【摘要】目的：探讨硫化氢（H2S）对兔离体心脏心率的影响。方法：用标准微电极细胞内记录技术，观测硫化氢对兔右心房心肌细胞动作电位频率的影响。观测指标：自发放电频率(rateofpacemakerfiring，RPF)。结果：(1)用200μmol/LNaHS灌流时，和正常对照组相比，RPF显著减小（P<0.01）。(2)400μmol/LNaHS与对照组比较，RPF显著减小（P<0.01）。和200μmol/LN

2、aHS相比，RPF显著减小（P<0.01）。(3)以上使RPF减小的效应在NaHS灌流3～5min出现，10min时已经有显著减小（P<0.01），25～30min达到高峰。结论：H2S可浓度依赖性的影响兔离体心脏的心率。【关键词】心率；电生理；硫化氢；心脏；兔Hydrogensulfide(H2S)hasbeenprovedtobethethirdendogenoussignalinggasotransmitter,besidesnitricoxide(NO)andcarbonmonoxide

3、(CO).Itshowsimportantphysiologicalfunctions,involvinginthelongtermpotentiationinthehippocampusinthecentralnervoussystem,relaxinggastrointestinaltractsmoothmuscles[1,2].H2Shasbeenalso11foundtoplaymanyregulatingfunctionsincardiovascularsystem,andplayacertai

4、neffectinmanycardiovasculardiseases[3].Buttheeffectonheartratehasntbeendeeplyreseached.ByusingstandardintracellularmicroelectrodetechniquetorecordAps,hydrogensulfidewasusedtoinvestigatetherateofpacemakerfiringofleftatrium.Theaimofthestudywastoobservetheef

5、fectsofhydrogensulfideonheartrateofrabbitheartsinvitro,elucidatethemechanism(s)involved,provideelectrophysiologicproofforthenosogenesisofsomecardiovasculardiseasesandprovideresearchdirectionsforthedevelopmentofcardiovasculardrugs.1MATERIALSANDMETHODS1.1Ani

6、malandReagentsRabbitsofeithersex(2.2±0.2)kg,providedbyExperimentAnimalCenterofHeBeiNorthUniversity.NaHSwaspreparedwithHClandNa2Saftertheexperimentbegan.1.2SpecimenPreparation11Rabbitswerekilledwithasingleblowontheheadandtheheartswerequicklyremovedandplaced

7、inwarm(36.0±0.5)℃improvedLockesolution(NaCl0.157mol/L,KCl0.56×10-2mol/L,CaCl0.21×10-2mol/L,NaHCO30.18×10-2mol/Landglycose0.56×10-2mol/L).Itwasoxygenatedwith95%O2and5%CO2andmaintainedat（36.0±0.5）℃withpHof7.40±0.03.Therightatriummyocytes(includingsinuatrialn

8、ode)werecutfromtherightatriumandthenpinnedonathinsilicondiscatthebottomofaperfusionchamber.Thepreparationswereperfused(5mL/min)withwarm(36.0±0.5)℃improvedLockesolution.Itwasoxygenatedwith95%O2and5%CO2andmaint