外文文献原稿和译文

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1、外文文献原稿和译文原稿Pharmacokineticstudyofsixflavonesinratplasmaandtissuesafteroraladministrationof‘JiangYaBiFeng’usingSPE-HPLC-DADAbstractInthisstudy,ahighperformanceliquidchromatography(HPLC)coupledwithdiodearraydetection(DAD)forsimultaneousdeterminationofsixflavonesincludingbaicalein,sophoricoside,r

2、utin,baicalin,quercetinandgenisteininratplasmaandtissuesafteroraladministrationofJiangYaBiFeng(JYBF)tabletswasdeveloped.Theinvestigatedanalytesinplasmaandtissueswereextractedandpurifiedwithliquid-liquidextractionandsolidphaseextraction(SPE).ChromatographicseparationwasaccomplishedonaDIONEXAccl

3、aimC18column(250mm×4.6mm,5.0μmparticlesize)withasimplelineargradientelution.ThecalibrationcurvesforalltheflavoneshadgoodlinearityinthemeasuredrangewithR2higherthan0.9983.Therelativeerrors(REs)oftheintra–andinter–dayaccuracyatdifferentflavoneslevelswerealllessthan±10%.Theproposedmethodenablesun

4、ambiguousidentificationandquantificationofinvestigatedflavonesinvivo.ThisisthefirstreportondeterminationofthemajorflavonesinratplasmaandtissuesafteroraladministrationofJYBFtablets.Theresultprovidedameaningfulbasisforevaluatingtheclinicalapplicationofthismedicine.Keywords:JiangYaBiFengtablet;Fl

5、avones;HPLC–DAD;Ratplasma;Tissuedistribution1.Introduction‘JiangYaBiFeng’tablet(JYBFtablet),anewdrugforthetreatmentofhypertensionhasbeenwidelyusedintheclinicinchinabecauseofitsgoodeffectandsmallsideeffects.Itiscomposedoftwowesternmedicines(pargylineand17hydrochlorothiazide)andthreetraditionalC

6、hinesemedicines(TCMs),includingScutellariabaicalensis,SophorajaponicaandArachishypogaea.Accordingtosomereportedpapers,flavones,suchasbaicalin,baicalein,sophoricoside,rutin,quercetinandgenisteinoriginallyfromthethreeTCMsarethemaineffectivecomponentscontainedinthisformulation.Becausethetherapeut

7、iceffectsofTCMsarebasedonthecomplexinteractionsofmultipleingredients,investigationofthepharmacokineticstudiesofmultiflavonesafteradministrationofJYBFtabletsisessentialtounderstandtheirroleinhumanhealthandevaluatetheclinicalefficacyofthi