硫酸铵饱和度的常用表,聚丙烯酰胺凝胶的配制表

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1、硫酸铵饱和度的常用表1．调整硫酸铵溶液饱和度计算表（0℃）在0℃硫酸铵终浓度，%饱和度硫酸铵初浓度，％饱和度 20253035404550556065707580859095100每100ml溶液加固体硫酸铵的克数010.613.416.419.422.625.829.132.636.139.843.647.651.655.960.365.069.757.910.813.716.619.722.926.229.633.136.840.544.448.452.657.061.566.2105.38.110.913.916.920.023.326.630.133.737.441.245

2、.249.353.658.162.7152.65.48.211.114.117.220.423.727.130.634.338.142.046.050.354.759.22002.75.58.311.314.317.520.724.127.631.234.938.742.746.951.255.72502.75.68.411.514.617.921.124.528.031.735.539.543.647.852.23002.85.68.611.714.818.121.424.928.532.336.240.244.548.83502.85.78.711.815.118.421.82

3、5.429.132.936.941.045.34002.95.88.912.015.318.722.225.829.633.537.641.84502.95.99.012.315.619.022.626.330.234.238.35003.06.09.212.515.919.423.026.830.834.85503.06.19.312.716.119.723.527.331.36003.16.29.512.916.420.123.127.96503.16.39.713.216.820.524.47003.26.59.913.417.120.97503/26.610.113.717

4、.48003.36.710.313.98503.46.810.59003.47.09503.510002．调整硫酸铵溶液饱和度计算表（25℃）在25℃硫酸铵终浓度，%饱和度硫酸铵初浓度，％饱和度 10202530333540455055606570758090100每1000ml溶液加固体硫酸铵的克数05611414417619620924327731335139043047251656166276710 578611813715018321625128832636540644949459269420 2959789112315518922526230034038242452061

5、925 3049619312515819323026730734839048558330 1930629412716219823527331435644954633 12437410714217721425229233342652235 31639412916420023827831941150640 31639713216820524528537546945 32659913417121025033943150 336610113717621430239255 336710314117926435360 346910514322731465 347010719027570 357

6、215323775 3611519880 7715790 793．不同温度下饱和硫酸铵溶液的数据温度（℃）010202530重量百分数41.4242.2243.0943.4743.85摩尔浓度3.93.974.064.104.13每1000g水中含硫酸铵摩尔数5.355.535.735.825.911000ml水中用硫酸铵克数706.8730.5755.8766.8777.5每1000ml溶液中含硫酸铵克数514.8525.2536.5541.2545.9聚丙烯酰胺凝胶的配制表1配制Tris-甘氨酸SDS-PAGE聚丙烯酰胺凝胶电泳分离胶所用溶液溶液成分不同体积（ml）凝胶液中各成

7、分所需体积（ml）5101520253040506%水2.65.37.910.613.215.921.226.530%丙烯酰胺溶液1234568101.5mol/LTris(pH8.8)1.32.53.856.37.51012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.048%水2.34.66.