磷酸盐缓冲液(pbs)配制方法

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1、磷酸盐缓冲液（PBS）配制方法科研实验 2010-04-2022:25:48 阅读935 评论0  字号：大中小 订阅0.01MPBS PBS(135mMNaCl,2.7mMKCl,1.5mMKH2PO4,and8mMK2HPO4，pH7.2)PBS缓冲液（pH7.2～7.4）：NaCl137mmol/L，KCl2.7mmol/L，Na2HPO44.3mmol/L，KH2PO41.4mmol/L称7.9gNaCl，0.2gKCl，0.24gKH2PO4(or1.44gNa2HPO4)和1.8gK2HPO4，溶于800ml 蒸

2、馏水中，用HCl调节溶液的pH值至7.4，最后加蒸馏水定容至1L。保存于4℃冰箱中即可。需要注意的是，通常所说的浓度0.01M指的是缓冲溶液中所有的磷酸根浓度，而非Na离子或K离子的浓度，Na离子和K离子只是用来调节渗透压的。母液的配制： 0.2MNa2HPO4：称取71.6gNa2HPO4-12H2O，溶于1000ml水0.2MNaH2PO4：称取31.2gNaH2PO4-2H2O，溶于1000ml水各种浓度PB(pH=7.4)的配制：先配0.2MPB(pH=7.4，100ml)：取19ml0.2mol/L的NaH2PO4

3、， 81ml0.2mol/L的Na2HPO4,即可。然后只需将0.2MPB(pH=7.4)按相应比例适当稀释即可，如：0.1MPB（PH=7.4）：取500ml0.2MPB，加水稀释至1000ml即可。0.01MPB（PH=7.4）：取50ml0.2MPB，加水稀释至1000ml即可。0.02MPB（PH=7.4）：取100ml0.2MPB，加水稀释至1000ml即可。 若需要NaCl的话，加入NaCl至0.9%（g/100ml）即可。另：其它各种另PH值的0.2MPB（100ml)配方：pH      0.2MNaH2PO

4、4（ml）        0.2MNa2HPO4（ml）5.7           93.5                         6.55.8             92                             8            5.9            90                             106.0            87.7                         12.36.1            85                  

5、          156.2             81.5                         18.56.3             77.5                         22.56.4            73.5                         26.56.5            68.5                         31.56.6            62.5                         37.56.7        

6、    56.5                         43.56.8            51                           496.9             45                           557.0             38                           627.1             33                          677.2            28                         

7、  727.3             23                           777.4             19ml                        81ml7.5             16                           847.6             13                           877.7            10.5                          90.57.8             8.5    

8、                     91.57.9            7                             938.0            5.3                         94.7 0.01M磷酸盐缓冲液（PBS）配制方法称取8gN