基质鲎试剂盒使用说明书(含偶氮)[]（matrix limulus kit use instructions (including azo) []）

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1、基质鲎试剂盒使用说明书(含偶氮)[1]（Matrixlimuluskituseinstructions(includingazo)[1]）Descriptionoftheuseofcolormatrixlimuluskit(endcolormethod,azo-azoreagent)[purpose]theChromogenicend-pointTachypleusAmebocyteLysate,CETALisusedforquantitativedetectionofbacterialendotoxininvitroandisforbidd

2、entoenterthebodyinanyway.【principle】thehorseshoereagentisafreeze-driedproductoflysisofthehaemolyticcellsoftheOrientalhorseshoecrabsofthehorseshoecrabsItcontainsCfactor,Bfactor,coagulaseandcoagulase.UndertherightconditionsDegree,pHandnointerferingsubstance,bacterialendotoxin

3、activatesCfactor,whichcausesaseriesofenzymaticreactions,Thecoagulasewasactivatedtoformthecoagulase,andthecoagulasewasdecomposedintothechromogenicmatrixPolypeptideandyellowp-nitroaniline(pNA,lambdaMax=405nm).Inacertainamountoftime,pNATheamountoftheproductispositivelycorrelat

4、edwiththeconcentrationofbacterialendotoxin.Accordingly,theconcentrationofendotoxincanbequantitativelytested.Atthesametime,thenitricaniline(pNA)isalsousedtodyetheredrosered(lambdaMax=545nm),Avoidtheinterferenceofthecolorofthetestproductitselftotheabsorptionpeakof405nm.Thedet

5、ectionlimitscanbedetectedintworegions:0.1EU/ml-1EU/mland0.01EU/ml-0.1EU/mlTheresponsetimeT1andT2seethefactoryinspectionreport.【composition】Bacterialendotoxinwork,2Limulusreagent,1.7ml/branch,2Colormatrix,1.7ml/branch,2Azochemicalreagent1,10ml/branch,2Diazochemicalagent2,10m

6、l/branch,2Azochemicalagent3,10ml/branch,2HCl(reactionterminationagent),50ml/bottle,1bottleBacterialendotoxintestwater,50ml/bottle,2bottles【storage】theshade,thebest2-8℃,avoidlightstorage.[usage]1.Materialsandequipment1.1reagentReagentoflimulusreagent,bacterialendotoxin,color

7、matrix,azochemicalreagent1,azochemicalreagent2,azotizationtestAgent3,HCl(reactionterminationagent),bacterialendotoxintestwater.1.2equipmentThereisnohottube,noheatsource.Page1Vortexmixer,pipette,multi-channelpipette,sealingfilm,testtuberack.Constanttemperaturewaterbath(37+/-

8、1℃),spectrophotometer.Note:allcontainersforcontactreagentsandsamplesmustbefreeofho