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1、EffectofsaffloryellowonplateletactivatingfactormediatedplateletactivationinpatientswithcoronaryheartdiseaseDaminHuang,YingminLu*,XiaohanLuo,LaixinShi,JinchunZhang,JunxianShen,MinminBao,LeiSong,CaiwenWei,HongsongLi,ZhihuaLiDepartmentofCardiology,Xinhua(Chongming)Hospital
2、ofShanghaiJiaotongUniversity,Shanghai202150,China*Correspondingauthor:YingminLu,DepartmentofCardiology,Xinhua(Chongming)HospitalofShanghaiJiaotongUniversity,Shanghai202150,China;Email:lilysweet111@sina.comAbstractTheplateletaggregationand5-HTreleasebywashedplatelet(WCP)
3、fromcoronaryheartdisease(CHD)patientsfollowingplateletactivatingfactor(PAF)treatmentweredetectedbyturbidimetryandO-phthalaldehyde(OPT)assay.Thefreecalciumconcentrationintheplateletswasmeasuredwiththefura-2/AMprobefluorescenttechnique.Resultsshowedsaffloryellowcouldinhib
4、itthePAFinducedWCPaggregationand5-HTrelease,whichwereinasafflor-yellow-dosedependentmanner.WhenthePAFwas2.0×10-9mol/L,theinhibitionrateofplateletaggregationwas26.2%,41.3%,58.1%,81.2%,andtheinhibitionrateof5-HTreleasewas3.7%,11.9%,29.9%and54.4%aftertreatmentwithsafflorye
5、llowat0.21,0.42,0.85and1.69g/L,respectively.ThestudyconcludesSaffloryellowcaninhibitthePAFinducedplateletaggregation,5-HTreleasebyplateletsandelevationoffreecalciuminplatelets.Keywords:Saffloryellow;plateletactivatingfactor;plateletaggregation;5-HT;freecalciuminplatelet
6、IntroductionSaffloweristhedryflowerofCathamustinctoriusLwhichhasbeenusedintraditionalChinesemedicineforpromotingbloodcirculationforremovingbloodstasis.Todate,ithasbeenappliedinthetreatmentoftrauma,gynecologicaldiseases,cardiovasculardiseasesandpulmonaryfibrosis[Wangetal
7、.,2011].Morethan200compoundshavebeenisolatedfromSafflowerincludingflavonoids,phenylethanoidglycosides,coumarins,fattyacids,steriodsandsafflowerpolysaccharides.Amongthesecompounds,saffloryellow(SY)istheeffectivepartoftheaqueousextractofsafflower[Fanetal.,2009].Hydroxysaf
8、floryellowA(HSYA)isanimportantactiveingredientofSYandsafflower.TheSYforinjectionhasbeenusedtotreatmyocardialis
