资源描述:
《大豆dreb转录因子的生物信息学分析》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、---------------------------------------------------------------范文最新推荐------------------------------------------------------大豆DREB转录因子的生物信息学分析摘要:大豆DREB类转录因子在逆境下对提高植株耐受性的具有重要作用。本研究从NCBI数据库中搜索大豆DREB转录因子序列,下载后对其基本特性如氨基酸序列组成、等电点、分子量、亲水/疏水性、信号肽、亚细胞定位、跨膜结构域、前导肽、磷酸化、蛋白紊乱区、球蛋白区、保守结构域及高级结构等方面进行预测和分析。结果表明,共获得2
2、0条大豆DREB转录因子,在N端都具有显著的AP2保守结构域,可分为6个小类群,进化分析分为4类;不同类DREB转录因子氨基酸长度以及基本性质差异较大,但均为水溶性蛋白,亲水性程度不同;序列中Cys含量均最少,均无跨膜结构,二级结构均以无规则卷曲为主。三级结构较为相似,9/9---------------------------------------------------------------范文最新推荐------------------------------------------------------均由一段α-螺旋和一组反向β-折叠结构组成。本研究为进一
3、步研究大豆DREB转录因子的结构及其功能提供了理论基础。关键词:大豆;DREB转录因子;生物信息学分析10659BioinformaticAnalysisofDREBTranscriptionFactorsofGlycinemaxAbstract:InordertoidentifytheroleofDREBtranscriptionfactorstoimprovethetoleranceofplantinstresscircumstance,theDREBtranscriptionfactorsofGlycinemax.wassearchedanddownloadedfromtheNCBIda
4、tabase,andtheirbasiccharterssuchasaminoacidcomposition,isoelectricpoint,molecularweight,hydrophilicity/hydrophobicity,signalpeptide,subcellularlocalization,transmembraneregion,leaderpeptide,phosphorylation,proteinglobulin,disorderedregion,conserveddomainsandseniorstructurewerepredictedandanalyzedby9
5、/9---------------------------------------------------------------范文最新推荐------------------------------------------------------bioinformaticwayandtechnology.Theresultsshowedthat,20ofDREBtranscriptionfactorshavebeengottentotally,withaconservedAP2domainintheN-terminus,whichpidedintosixsmallgroups,and4cl
6、assesbyevolutionaryanalysis.TheaminoacidlengthandthebasicpropertiesofeachclassofDREBtranscriptionfactoraredifferent;theyareallwater-solubilityproteins,hydrophilicdegreearedifferent,Ofeachsequence,thenumberofCyswasthefewest,therewasnotransmembranestructure,randomcoilisthemainmodalityofthesecondarystr
7、ucture.ThetertiarystructureoffourDREBtranscriptionfactorsinsoybeanareverysimilarandcomposedofanalphahelixandasetofreversebetafoldingstructure.Thestudylaidtheoreticalfoundationsforfurtherstudythestruct