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1、大鼠耳科常规手术径路李鹏1,2丁大连1,2,3高可雷2,3RichardSalvi2,31中山大学附属第三医院耳鼻咽喉-头颈外科2CenterforHearingandDeafness,UniversityatBuffalo3中南大学湘雅医院耳鼻咽喉头颈外科第一作者：李鹏通讯作者：丁大连E-mail:dding@buffalo.edu课题资助：1、NationalInstituteforOccupationalSafetyandHealth：R01OH010235,2、广东省科技计划项目：2011B0318003593、国家外国专家局审批类项目：CG2013440003摘要目的:
2、介绍几种在大鼠耳科实验研究中常用的手术方法。方法:将大鼠麻醉后，根据不同的实验需求，分别演示经耳后切口的后鼓室径路、经耳上切口的上鼓室径路、经颈部腹侧切口的下鼓室径路、经颈部背侧切口的枕后径路等手术方法。结果：通过不同的手术径路可从不同的角度暴露大鼠的中耳和内耳，为引导耳蜗生物电反应的各种耳蜗电极置放、经圆窗膜转染或置药、药物经耳蜗外淋巴腔灌流、耳蜗内淋巴腔灌注、听骨链中断、以及内淋巴囊手术破坏等各种不同实验目的提供最佳手术方案。结论：根据大鼠颞骨解剖特点，选择不同的中耳内耳手术径路，可以为实现不同的实验目的服务，对实验成功提供技术保障。关键词:大鼠,中耳,内耳,手术径路Stan
3、dardsurgicalapproachesoftheearsurgeryinratsPengLi1,2DalianDing1,2,3KeleiGao2,3RichardSalvi2,31TheThirdAffiliatedHospitalofSunYat-SenUniversity122CenterforHearingandDeafness,UniversityatBuffalo3XiangyaHospital,CentralSouthUniversitAbstractObject：Tointroduceseveralotologicsurgicalapproachesfors
4、pecificexperimentalstudiesinrats.Methods:Basedonthespecificgoalsoftheexperiment,anesthetizedratswerepreparedfortheearsurgeryusingdifferentsurgicalapproachesaimedatoptimizingtheexperimentaloutcome.Thesesurgicalapproachesincluded:posteriortympanum,superiortympanum,inferiortympanum,andoccipitals
5、urgeries.Result:Themiddleearcavityandtheinnerearcanbeexposedfromdifferentanglesbyusingdifferentsurgicalapproaches.Forexample,electrodeplacementforrecordingofcochlearbioelectricresponsescanbeachievedusingapproachesthroughtheposteriortympanumorinferiortympanum.Alternatively,drugdeliveryorgenetr
6、ansfectionviaroundwindowmembranecanbemosteasilyaccomplishedusingasurgicalapproachfromtheposteriortympanum.Cochlearperfusionofprotectiveorototoxicdrugcanbebestperformedusingtheinferiortympanumapproach.Ossicularchaininterruptiontoinduceaprolongedconductivehearinglosscanbereadilyachievedusingasu
7、periortympanumapproach.Lastly,surgicaldestructionofendolymphaticsactoinduceexperimentalendolymphatichydropscanbereadilyperformedfromanoccipitalsurgicalapproach.Conclusion:Thesestandardsurgeriescanbeappliedforscientificstudiesoftheearwithdiffe
