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1、广西45个主栽水稻品种抗性基因遗传多样性分析南'农业2011,42(10):1173—1176JournalofSouthernAgricuhure广西45个主栽水稻品种抗性基因遗传多样性分析张晋..,罗志勇,颜群,钟波,高利军,潘筱凌.,赖海燕,黄鹂,高汉亮,郭小强(.广西农科院植物保护研究所,南宁530007;广西作物遗传改良生物技术重点开放实验室,南宁530007广西区种子管理总站.南宁530022)摘要:【目的】明确近年来广西45个主要栽培水稻品种的抗性基因遗传多样性,揭示其遗传差异,为更好筛选水稻品种组合和通
2、过品种间轮作及合理布局控制病害提供理论依据.【方法】根据已克隆的抗病基因设计5对RGA引物,对广西45个主栽水稻品种进行抗病基因同源序列分析并构建聚类图谱.【结果】在45个水稻品种的抗病基因中,共扩增出813条带,其中多态性条带124条,多态率达76.07%.聚类结果显示,不同品种间遗传相似系数变幅为0.61—0.93,取相似系数0.67时,可将45个水稻品种分为5大类,聚类结果在一定程度上反映品种来源的地域性和亲缘关系.【结论】广西不同主栽水稻品种间抗病基因数量差异较大,在广西稻瘟病发生严重的地区,采取不同抗性遗传背
3、景的水稻品种轮作及混栽是控制该病害最为有效的措施之一.关键词:水稻;主栽品种;抗病基因同源序列;遗传背景;广西中图分类号:S511文献标志码:A文章编号:2095一l191(2011)10—1173—04Geneticdiversityamongst45mainricecultivarsinGuangxiZHANGJin一,LUOZhi—yong,YANQun,ZHONGBo.,GAOLi-jun一,PANXiao—ling,LAIHai—yan,HUANGLi.,GAOHan—liang,GUOXiao—qiang(
4、PlantProtectionInstitute,GuangxiAcademyofAgricultureSciences,Nanning530007,China;GuangxiCropGeneticImprovementandBiotechnologyLab,Nanning530007,China;GuangxiSeedsManagementStation,Nanning530022,China)Abstract:【Objective]Thepresentexperimentwasconductedtoassessth
5、egeneticdiversityinrelationtogeneticvari—ationsindiseaseresistanceamongst45mainricecuhivarsofGuangxi.【Method】FivepairsofRGAprimersweredesignedonthebasisofalreadyidentifieddiseaseresistantgenesinordertofindoutthepresenceorabsencein45ricecultivars.【Result]Total813
6、bandswereamplifiedfrom45ricecultivars,ofwhich124werepolymorphicandthepolymorphicratewas76.07%.Theclusteranalysisresultshowedthatthesimilaritycoefficientofthesecuhivarsrangedfrom0.61to0.93andthesecuhivarsweredividedinto5classificationsat0.67.Italsorevealedthepres
7、enceoffivedistinctindicatingregionalismanddistantgeneticrelationshipsamongstricecuhivarsources.【Conclusion】Significantgeneticvariationshavebeenob—servedinresistancetovariousdiseases.Cultivarrotationandplantingofmixed—cuhivarswithdifferentgeneticbackgroundshavebe
8、ensuggestedasaneffectivewayforcontrollingriceblastdiseaseinGuangxi.Keywords:rice;cuhivars;homologicalsequenceofresistantgenetodisease;geneticbackground;GuangxiO5I昂【研究
