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1、厚皮甜瓜叶片展开至衰亡过程中保护酶活性及膜脂过氧化的变化●CT-4ilRICULTURAEBOREaLI-SIHIrdl华北农?2OO7,22(5):93-96厚皮甜瓜叶片展开至衰亡过程中保护酶活性及膜脂过氧化的变化王冰林,李媛媛,何启伟.,刘世琦4,焦自高.,李芳贤(1.山东省潍坊市农业科学院,山东潍坊261061;2.潍坊学院生物工程学院,山东潍坊261061;3.山东省农业科学院蔬菜研究所,山东济南250100;4.山东农业大学园艺科学与工程学院,山东泰安271018)摘要:以目光温室栽培的伊丽莎自和蜜
2、世界厚皮甜瓜为试材,通过测定不同叶龄叶片超氧化物歧化酶(SOD),过氧化物酶(POD),过氧化氢酶(CAT)活性和丙二醛(MDA)含量,探讨了在叶片展开至衰亡过程中保护酶活性和膜脂过氧化变化.结果表明,在叶龄7～21d,SOD活性不断升高,之后短时下降,在叶龄28～35d(伊丽莎自;蜜世界延续到42d).酶活性迅速升高,随后急剧下降.POD,CAT活性和MDA含量均呈"先升后降"的变化趋势:伊丽莎自,蜜世界CAT活性上升分别从7d叶龄持续到21d和28d,之后不断降低;而POD活性上升则分别持续到49d和56
3、d叶龄,随后大幅下降;MDA含量与POD活性变化基本一致.在叶片衰老期间,中晚熟品种SOD和CAT活性显着高于早熟品种,而POD活性和MDA含量则明显偏低.关键词:厚皮甜瓜;叶片展开;叶片衰亡;保护酶活性;膜脂过氧化中图分类号:S652.0l文献标识码:A文章编号:1000—7091(2007)05—0093—04ChangesoftheActivitiesofProtectiveEnzymesandSuperoxidationofMembraneLipidinMuskmelonLeavesDuringUnf
4、oldingandContabescenceWANGBing—lin,LIYuan—yuan2,HEQi—wei,LIUShi—qi,JIAOZi—gao,LIFang—xian(1.WeifangAcademyofAgricultureSciences,Weifang261061,China;2.DepartmentofBioengineering,WeifangAcademy,Weifang261061,China;3.VegetableResearchDepartment.ShandongAcademy
5、ofAgricultureSciences,Jinan250100,China;4.CollegeofHorticultureScienceandEngineering,ShandongAgriculturalUniversity,Taian271018,China)Abstract:Changesoftheactivitiesofprotectiveenzymesandsuperoxidationofmembranelipidinleavesduringan—foldingandcontabescencew
6、erestudiedusingthemuskmelonvarietiesofHoneyWorldandYilishabaigrowninsolargreenhousebymensuratingtheactivitiesofsuperoxidedismutase(SOD),peroxidase(POD)andcatalase(CAT),andthecontentofmalondialdehyde(MDA).TheresultsindicatedthattheSODactivityincreasedcontinu
7、ouslyduring7～21dofleafageanddecreasedatshortnotice;andtheSODactivityofYilishabaiincreasedrapidlyduring28～35dwhileitin—creaseduntil42dforHoneyWodd.Subsequently,itdeclinedsharply.rheactivitiesofPODandCAT,andthecontentofMDAallincreasedatfirstbutdeclinedwhereaf
8、ter.TheactivitiesofCATrisedfrom7dto21dand28dofleafageforYilishabaiandHoneyWorld,respectively,anddeclinedcontinuouslywhereafter.TheactivitiesofPODrisedfrom7dto49dand56dofleafageforthetwovarieties,respec
