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1、HBV转基因小鼠胚胎玻璃化冷冻保存研究2005年6月第l5卷第3期中国比较医学杂志CHINESEJOURNALOFCOMPARATIVEMEDICINEJune,2005V01.15No.3,,口,口e研究报告$蛤,蛤畸,HBV转基因小鼠胚胎玻璃化冷冻保存研究尚书江,孔德强,王冬平,张爱兰,曾(1.军事医学科学院实验动物中心,北京3.中国农业大学动物科技学院,北京林,朱士恩,杨晓,孙岩松100071:2.军事医学科学院生物工程研究所;100094;4.吉林大学农学部,长春130062)【摘要】目的研究OPS法对不同基因型的HBV转基因小鼠胚胎玻璃化冷冻的效果,为建立
2、转基因小鼠胚胎库奠定基础.方法采用乙二醇作为冷冻保护剂,以野生型P2小鼠为对照,用OPS法对P2及P1,HBBx两种HBV转基因小鼠3.5d的胚胎进行玻璃化冷冻,并比较不同基因型小鼠的超数排卵数,胚胎的复苏率及发育率.结果,",P2#HBsAs及P2小鼠平均超排数分别为27.89,15.56,9.14枚;复苏率分别为88.7%,81.1%,80.3%;发育率分别为82.6%,79.4%,56.6%.结果表明HBV转基因小鼠的超排数明显低于野生型小鼠的超排数,解冻后三种小鼠的胚胎复苏率之间无显着差别,而解冻后发育率,P2小鼠要明显低于其它两种小鼠.结论超排数,胚胎复苏
3、率及解冻后发育率受小鼠的基因型影响.【关键词】HBV;转基因;小鼠胚胎;玻璃化冷冻【中图分类号】Q95—331【文献标识码】A【文章编号】1671—7856(2005)03—0139—04CryopreservationofEmbryosofHBVTransgenicMiceinaVitrificationSolutionSHANGShu—jiang,KONGDe—qiang,WANGDong—ping,ZHANGAi—lanZENGLin4,ZHUShi—en,YANGXiao2,SUNYan.song(1.LaboratoryAnimalCenter;2.Med
4、icalEngineeringInstitute,MilitaryAcademyofMedicalSciences,Beijing100071,China;3.CollegeofAnimalSciencesandTechnology,ChinaAgriculturalUniversity,Beijing100094,China;4.JilinUniversityAgriculturalCollege,Changchun130062,China)【Abstract】ObjectiveInordertoevaluatethevalueofopenpulledstraw(
5、OPS)methodoncryopreservationofembryosofdifferentgenotypesofHBVtransgeniemice,andtoprovideabasisforestablishmentoftransgenieembryosbank.MethodsEth—yleneglygol(EG)wasusedasthecryoprotec~ntforeryoprese~ationofmorulaandblastocystembryosofP21…,P21"andP21"".ResultTheaveragenumbersofembryopro
6、ductionofP21,P21№№^暑andP21HBB1were27.89,15.56and9.14,respectively,aftersuperovulatorytreatment.TherecoveryratesofP21,P21"^暑andP21呻洲B1were88.7%,81.1%and80.3%.respectively.Thedevelopmentratesafterthawingwere82.6%,79.4%and56.6%invitro,respectively.Theresuitsindicatedthatthenumbersofsupero
7、vulationofP'1"矗^HBsAandP21呻"BlwerelowerthanthoseofP21.TherecOV—eryrateswerenotsignificantlydifferentinthethreegenotypesmice.ThesurvivalrateafterthawingofP21waslowerthanthat0fP21andP21".ConclusionThegenotypeofmiceaffecttheresultsofsuperovulationnumber,recoveryrateandsur—vivalofembryos
