糖尿病心肌病及早期扩张性心肌病心肌线粒体通透性转运孔开放对比观察

《糖尿病心肌病及早期扩张性心肌病心肌线粒体通透性转运孔开放对比观察》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、糖尿病心肌病及早期扩张性心肌病心肌线粒体通透性转运孔开放对比观察【摘要】目的：观察糖尿病心肌病与早期扩张性心肌病心肌线粒体通透性转运孔开放程度异同。方法：30只Wistar大鼠随机分成对照组、糖尿病组及扩心病组。造模成功，提取心肌线粒体，检测线粒体PTP开放、膜电位及Ca2+转运。结果：MPTP开放检测吸光值糖尿病组最高，与对照组及扩心病组比较均有显著性差异，扩心病组与对照组比较，有显著性差异；Ca2+转运检测吸光值及膜电位Rh123荧光值糖尿病组与对照组比较，有显著性差异，扩心病组与对照组比较，有显著性差异。结论：糖尿病心肌病与早期扩张性心肌病线粒体MPTP呈高开

2、放状态，前者显著高于后者，MPTP呈高开放状态可能是糖尿病心肌病与早期扩张性心肌病在线粒体能量代谢方面共同病理生理机制。【关键词】糖尿病心肌病；扩张性心肌病；线粒体通透性转运孔DiabeticcardiomyopathyanddilatedcardiomyopathyearlymyocardialmitochondrialpermeabilitytransitionporeopeningComparativestudy【Abstract】Objective：Observationofearly8diabeticcardiomyopathyanddilatedcard

3、iomyopathymyocardialmitochondrialpermeabilitytransitionporesimilaritiesanddifferencesbetweenthedegreeofopenness。Methods：30Wistarratswererandomlydividedintocontrolgroup,diabetes,expandingheartdiseasegroup，Diabeticcardiomyopathyanddilatedcardiomyopathyearlymodelingsuccess,Detectedmitocho

4、ndrialPTPopeningandmembranepotentialandCa2+transport。Results：MPTPopeningdetectthehighestabsorbancevalueofthediabeticgroupcomparedwiththecontrolgroupandextendheartdiseasegroupweresignificantlydifferent,expandingheartdiseasegroupandcontrolgroup,thereisasignificantdifference；DetectionofCa

5、2+transportabsorbanceandMembranepotential,Rh123fluorescencediabeticgroupandcontrolgroup,thereisasignificantdifference,expandingheartdiseasegroupandcontrolgroup,thereisasignificantdifference。ConclusionDiabeticcardiomyopathyanddilatedcardiomyopathyearlymitochondrialMPTPshowedahighopensta

6、te,theformerwassignificantlyhigherthanthe8latter。Diabeticcardiomyopathyanddilatedcardiomyopathyearlyinthemitochondrialenergymetabolismthatisacommonpathophysiologicmechanism。【Keywords】Diabeticcardiomyopathy;dilatedcardiomyopathy;MPTP线粒体膜通透转运孔(mitochon-drialpermeabilitytransitionpore,MPT

7、P)是线粒体内外信息交流的中心枢纽,其开放状态决定着线粒体功能的发挥。本研究旨在观察MPTP在糖尿病心肌病(diabeticcardiomyopathy,DC)及扩张性心肌病实验大鼠中(dilatedcardiomyopathy,DCM)开放高低的差异，以探讨MPTP在两者病理生理机制中作用的异同。1材料和方法1.1材料1.1.1动物清洁级雄性Wistar大鼠40只,体重180~200g,由华中科技大学实验动物中心提供(合格证001号,环境设施合格证028号)。随机分为对照组、糖尿病心肌病组（糖尿病组）、扩张性心肌病组（扩心病组），每组10只。81.1.2试剂链