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1、欧美山杨杂种嫩枝微扦插生根相关氧化酶活性变化及繁殖技术第39卷第11期2011年11月东北林业大学JOURNALOFNORTHEASTF0RESTRYUNIVERSITYVo1.39No.1lNov.2Ol1欧美山杨杂种嫩枝微扦插生根相关氧化酶活性变化及繁殖技术)闫绍鹏武晓东王秋玉宋兴舜杨瑞华(东北林业大学,哈尔滨,150040)(黑龙江省农业科学院园艺分院)摘要选用外源激素种类(NAA,IBA,I从),激素质量浓度和处理时间3个因素,采用(3)正交设计研究了欧关山杨杂种(PopulustremulaxP.tremuloides)嫩枝微扦插繁
2、殖技术,以及生根过程中过氧化物酶(POD),多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)的活性变化.结果表明:外源激素种类对扦插生根率影响最大,其次是激素的质量浓度和处理时间.不同外源激素种类,激素质量浓度间生根率差异显着.所有处理中,NAA的质量浓度为100mg?L处理的插穗生根率最高,达到76%.欧关山杨杂种嫩枝微扦插生根过程可分为愈伤组织形成期,生根诱导期和表达期3个阶段.PCD活性在愈伤组织形成期,不定根诱导期呈上升趋势,在生根表达期下降;IAAO活性在愈伤组织形成期,不定根诱导期上升,在生根表达期保持较高活性,而在很生长后期下降
3、;PPO活性在愈伤组织形成期和不定根诱导期上升,在表达期下降.关键词欧美山杨杂种(PopldustremulaxP.tremuloides);微扦插;繁殖技术;氧化酶分类号$723.13ChangesinRelevantOxidaseActivitiesDuHngtheRootingofCuttingsofPopulustremulax只tremuloidesandCuttingPropagationTechnique/YanShaopeng,Wu~aMong,WangQiuyu,SongXingshtm(CollegeofLifeScien
4、ces,NortheastForestryUniversity,Harbin150040,P.R.China):YangRuihua(HorticulturalBranch.HeilongjiangAcademyofAcuhuralSciences)//JournalofNortheastForestryUniversity.一2011,39(11).一5～7.1lThecuttingpropagationtechniqueandrelevantoxidasechangesofPopulustremula×P.tremuloidesweres
5、tudiedwithaL.(3)orthogonalexperimentdesi~-mwhichconsistedofthreefactor8ofvarieties.aHxins(NAA.IBA,nA).auxinconcentrationsofandtreatmenttime.Theactivitiesofperoxidase(POD).polyphenoloxidase(PP0)andindoleacetieacidoxidase(IAAOduringtherootingperiodofcuttingwereanalyzed.Result
6、sshowedthatauxintypehadthemostobvi.OllSeffectontherootingrateofthecuttings,followedbyauxinconcentrationandauxintreatmenttime.Thereweresignificantdifferencesinrootingrateofthecuttingsbetweendiirerentauxintypesorauxinconcentrations.Amongallthetreat.merits.thecuttingstreatedwi
7、th100mg?LofNAAshowedthehighestrootingrate(76%).TherootingofthecuttingsP.tremulaxP.tremuloidescouldbeclassifiedintothreeperiods.namelyformationperiodofcallus.rootinductionpe.riod.androotexpressionperiod.TheP0Dactivityincreasedduringthecallusformationandinductionphases,butde—
8、creasedduringtheexpressionphaseoftherootingunderNAAtreatment.PPOactivityincreasedb
