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时间:2018-03-05
《工业化uasb厌氧颗粒污泥产氢产乙酸菌群分析》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、第45卷第3期Jou浙rnalof江Zhejia大ngUn学iversit学y(En报ginee(r工ing学S版cien)ce)VoI.45NO.32O11年3月Mar.2011DOI:10.3785/j.issn.1008—973X.2011.03.030工业化UASB厌氧颗粒污泥产氢产乙酸菌群分析马俊科,刘春,吴根,杨景亮,郭建博,李再兴(1.河北科技大学环境科学与工程学院,河北石家庄050018;2.科技部基础研究管理中心,北京100862)摘要:合成特异性探针,应用荧光原位杂交(FISH)技术分析阿维菌素废
2、水处理工业化升流式厌氧污泥床(UAsB)反应器颗粒污泥产氢产乙酸菌群分布和相对丰度,并测定菌群活性.结果表明:不同形成阶段颗粒污泥表面和内部剖面,产氢产乙酸菌、食丙酸盐产氢产乙酸菌和食丁酸盐产氢产乙酸菌的分布形态相同;产氢产乙酸菌平均相对丰度范围为(10.08-+-0.81)~(28.06±2.12).成熟期颗粒污泥中产氢产乙酸菌相对丰度最大;颗粒污泥表面产氢产乙酸菌相对丰度大于内部剖面;食丙酸盐产氢产乙酸菌相对丰度大于食丁酸盐产氢产乙酸菌.阿维菌素残留对产氢产乙酸菌群具有抑制作用.不同形成阶段颗粒污泥最大比产乙酸速
3、率范围为0.912~1.145g/(g·d),且与产氢产乙酸菌群相对丰度变化趋势一致.关键词:厌氧颗粒污泥;产氢产乙酸菌群;荧光原位杂交(FISH);相对丰度;阿维菌素废水中图分类号:X176文献标志码:A文章编号:1008—973X(2011)03一O576—06Analysisofsyntrophicacetogenicbacteriacommunityinanaerobicgranularsludgefromafull’scaleUASBMAJun—ke,LIUChun,WUGen,YANGJing—liang
4、,GUOJian_bo,LIZai—xing(1.SchooloJEnvironmentalScienceandEngineering,HebeiUniversityoJScienceandTechnology,S甜^“g050018,China;2.BasicResearchServiceCenter,theMinistryofScienceandTechnology,Beijing100862,China)Abstract:Probesspecificforsyntrophicacetogenicbacteria
5、weredesignedandfluorescenceinsituhybrid—ization(FISH)wasusedtoanalyzetheirdistributionandrelativeabundanceinanaerobicgranularsludgeinafull—scaleup—flowanaerobicsludgebed(UASB)reactortreatingavermectinwastewater,andtheactivityofsyntrophicacetogenicbacteriawasals
6、odetermined.Theresultsindicatedthatthedistributionformsofsyntrophicacetogenicbacteria,syntrophicpropionate—oxidizingbacteriaandsyntrophicsfattyacid一8一oxidi—zingsyntrophicbacteriaweresameonthesurfaceandinnerfaceofgranularsludgesamplesindifferentfor—mationperiods
7、.However,theaveragerelativeabundancesofthosesyntrophicacetogenicbacteriapopula—tionsweredifferent,rangingfrom(10.08±0.81)to(28.064-2.12).Themaximumrelativeabun—danceofsyntrophicacetogenicbacteriawasobtainedwhengranularsludgewasinmatureperiod.Therela—riveabundan
8、cesofsyntrophicaeetogenicbacteriaO171theinnerfacewerelessthanthoseonthesurfaceofgranularsludgesamples.Therelativeabundancesofsyntrophicpropionate—oxidizingbacteriawerelarger
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