叶用芥菜小孢子离体培养条件.pdf

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1、浙江农林大学学报，2014，31(2)：315—321JoitrnalofZhejiangA&FUniversitydoi：10．118336．issn．2095—0756．2014．02．023叶用芥菜小孢子离体培养条件吴慧敏，何勇，邵泱峰，朱祝军，符庆功(1．浙江农林大学浙江省农产品品质改良技术研究重点实验室，浙江临安311300；2．浙江省临安市农业技术推广中心，浙江临安311300)摘要：以5个叶用芥菜Brassicajunceavar．foliosa品种为试材，研究了游离小孢子培养过程中基因型、低温预处理、高温热激、更新及加液和秋水仙碱等对小孢子胚状体发生的影响。结果表明：有4个品

2、种获得胚状体，各品种间胚状体产量存在显著差异，‘特选九心芥’胚产量最高；4℃低温预处理24~48h及33~34qC高温热激处理24~48h对诱导胚状体发生和发育有促进作用；更新和添加培养基处理也可促进胚状体发生，以质量浓度为170．0g·L的蔗糖起始培养48h后添加等质量浓度90．Og·L蔗糖培养基处理效果最佳；1~10mg。L秋水仙碱24~48h处理可提高小孢子培养反应，增加供试材料胚产量。图1表5参19关键词：园艺学；叶用芥菜；小孢子培养；胚状体；热激；秋水仙碱中图分类号：$637．2文献标志码：A文章编号：2095—0756(2014)02-0315—07Invitromicrospo

3、recultureofleafmustardWUHuimin，HEYong，SHAOYangfeng，ZHUZhujun，FUQinggongf1．TheKeyofLaboratoryforQualityImprovementofAgriculturalProductsofZhejiangProvince，ZhejiangA&FUniversity．Lin’an311300，Zhejiang，China；2．AgriculturalTechnologyPromotionCenterofLin’anCity，Lin’an_311300，Zhejiang，China)Abstract：Inord

4、ertoimprovetheisolatedmicrosporeculturesysteminBrassicajunceavar．foliosa，fivedifferentgenotypesofBrassicajunceavat．foliosawereusedtoinvestigatethecontributionofgenotype，cool—pre—treatmentheatshock，mediumreplacementandaddition，aswellasconcentrationofcolchicineonmicrosporeembryogenesisinvitro．Results

5、showedthatembryoidswereobtainedfromfourgenotypes，andsignificantdif-ferences(P<0．05，Duncan’SMRT)wereobservedforembryoyieldwiththehighestyieldbeing‘Texuan—ji—uxin’．Forallcuhivars．24—48hofc0o1．pretreatmentat4℃andheatshockat33—34oCimprovedembryoyields．Also，mediumadditionandreplacementenhancedmicrospore

6、embryogenesiswiththebesttreatmentUS—ingaconstantvolumefora90．0g·L～sucrosemediumsupplementedtoa170．0g‘L一sucroseconcentrationmediumafter48h．Inaddition．treatingthefreshlyisolatedmicrosporewithl一10mg·L一colchicinefor24-48hgreatlyimproved(P<0．05，Duncan’sMRT)embryoyieldsofthefourcuhivars．Thepresentresults

7、couldbeusedtoobtainhaploideficientlyfortheplantbreedingandbasicresearchinBrassicaj．nceavar．foliosa．[Ch，l6g．5tab．19ref．]Keywords：horticulture；leafmustard；microsporeculture；embryoid；heatshock；colchicine叶用芥菜Br