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时间:2020-04-23
《人体带绦虫的分子鉴定-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、第39卷第3期贵阳医学院学报VO1.39No.32014年6月JOURNALOFGUIYANGMEDICALCOLLEGE2014.6人体带绦虫的分子鉴定:l=周灵贵,鞠伟,黄江,廖兴江,刘玉江,申萍香,戴佳琳(1.贵阳医学院多媒体形态学实验室,贵州贵阳550004;2.贵阳医学院法医学系贵州贵阳550004;3.贵阳医学院物理学教研室贵州贵阳550004)[摘要]目的:对采自云南大理的6条人体带绦虫进行虫种分子鉴定。方法:对云南省大理有排节片史的病人以槟榔一南瓜子法驱虫,对6条人体带绦虫成虫进行形态学鉴定;提取虫
2、体基因组DNA,用亚洲带绦虫和牛带绦虫线粒体coxl基因片段的特异性引物对DNA进行常规PCR扩增,扩增产物经琼脂糖凝胶电泳检测;将虫卵喂食2头幼猪(5x10/头),40d后解剖检查.观察囊尾蚴寄生情况。结果:6条人体带绦虫的形态和牛带绦虫相似,亚洲带绦虫coxl片段PCR为阳性,扩增产物约260bp,牛带绦虫coxl片段PCR均为阴性;只在感染幼猪肝脏发现有囊尾蚴寄生,其他组织和肌肉内没有囊尾蚴寄生。结论:形态学和分子生物学鉴定6条人体带绦虫标本均属于亚洲带绦虫。[关键词]亚洲带绦虫;牛带绦虫;形态学;分子生物学
3、;鉴定[中图分类号]R383.32[文献标识码]A[文章编号]1000-2707(2014)o3311-04MolecularIdentificationofHumanTaeniaTapewormsZHOULinggui,JUWei,HUANGJiang,LIAOXingjiang,LIUYujiang,SHENPingxiang,DAIJialin(』.MultimediaLaboratoryofMo~hology,GuiyangMedicalCollege,Guiyang550004,Guizhou,China
4、;2.DepartmentofForensicMedicine,GuiyangMedicalCollege,Guiyang550004,Guizhou,China;3.DepartmentofPhysiology,GuiyangMedicalCollege,Guiyang550004,Gu~hou,China)[Abstract]Objective:Toidentifythespeciesof6humanTaeniatapewormsinDaliofYunnanprov—ince.Methods:SixTaenia
5、tapewormswereexpelledandcollectedfrompatientswithproglottidsinstoolbyusingbetelnut—pumpkinseedmethodinDaliofYunnanprovince.Morphologicalidentifica·tionwasca~iedoutonthe6adultTaeniatapeworms.GenomicDNAofthe6wormswasextracted,andspecificprimersofmitochondrialcox
6、lgenefragmentsofTaeniaasiaticaandTaeniasaginatawereusedtoamplifyDNAwithconventionalPCR,andtheproductswereconfirmedbyagarosegelelectro—phoresis.Twopigswerefedwitheggsoftheworms(5X10’perpig).Anatomicalexaminationwasperformedonthe40day.Cysticercusparasitizingsitu
7、ationwereobserved.Results:Themorphologyof6Taeniatapewormswassimilartosaginata.ThePCRproductsusingasiaticafragmentsasprimerswerepositiveinelectrophoresisandwereabout260bp.Allsamplesusingsaginatafrag-mentsasprimerswerenegativeinelectrophoresis.Cysticercuswasfoun
8、dinliversoftheinfectedpigsratherthaninothertissuesandmuscle.Conclusions:Morphologicalandmolecularclassificationshowsthatthe6Taeniatapewormsareasiatica.[Keywords]Taeniaasiatica;Taen
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