圆头精子受精能力、胚胎发育潜能及遗传风险的研究

《圆头精子受精能力、胚胎发育潜能及遗传风险的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、中华医学杂志2010年9月7日第90卷第33期NatlMedJChina，September7，2010，V01．!．·2351·．临床研究．圆头精子受精能力、胚胎发育潜能及遗传风险的研究黄东蒋凌英徐维海童晓嵋竺海燕李超周枫刘柳林小娜张松英【摘要】目的探讨圆头精子的受精能力、胚胎发育潜能及其遗传风险。方法通过精子形态染色和透射电镜证实一例圆头精子症患者，其女方共获卵26颗，赠卵6颗，将20颗卵子(19颗为MII卵)行卵细胞质内单个圆头精子的显微注射，6颗赠卵行常规体外授精。测定该患者的染色体核型，同时针对Y染色体AZFa、AZFb、AZFc的sY84、sY86、sY127、s

2、Y134、sY254、sY255等6个序列标签位点(STS)进行Y染色体微缺失测定。结果19颗MII卵子行圆头精子显微注射后有4颗正常受精并卵裂，其余均未受精和卵裂。6颗赠卵常规体外授精后全部正常受精并卵裂，受精率显著高于圆头精子(100％VS21．1％，P<0．01)，卵裂率两者差异无统计学意义(100％VS100％，P>0．05)。患者染色体核型正常(46，XY)，未发现微缺失。女方行冻胚移植后妊娠，足月剖宫产一男孩，染色体核型正常(46，XY)。受者行冻胚移植后妊娠，足月剖宫产一男孩，体健。结论尽管受精率低下，卵细胞质内单精子注射(ICSI)仍不失为治疗圆头精子症患者不

3、育的一种有效方法。【关键词】精子；顶体；胚胎发育；Y染色体；染色体缺失Fertilizingability，cleavagepotentialandinheritanceriskofglobozoospermiaHUANGDong，JIANGLing—ying。XUWei—hai．TONGXiao—mei，ZHUHal—yanLIChaoZHOUFeng。LIULiuLlNXiao—na．ZHANGDepartmentofObstetrics&Gynecology，SirRunRunShawHospital，SchoolofMedicine&SirRunRanShawInst

4、ituteofClinicalMedicine，ZhefiangUniversity，Hangzhou310DJ6，ChinaCorrespondingauthor：ZHANGs0一ying，Email：zhangsongying@126．corn【Abstract】0bjectiveToexplorethefertilizingability，cleavagepotentialandinheritancerisksofglobozoospermia．MethodsAglobozoospermicpatientwasdiagnosedbyspermmorphologicals

5、tainingandtransmissionelectronmicroscope．Fromhiswifetheinvestigatorsobtained26oocytesinwhich6oocytesweredonatedandtheother19(in20)MIIOOCytesinjectedinto19round—headedsperms．Sixdonatedoocytesacceptedinvitrofertilization(IVF)．r}lispatientschromosomeandmicrodeletioninAZFa．AZFbandAZFcinYchromos

6、omewerecheckedthrouIgh6sequencetagsitesofsY84，sY86，sY127，sY134，sY254andsY255．ResultsOnly4(in19)werenormallyfertilizedandcleaved．A116donatedoocyteswerenormallvfertilizedandcleaved．Thefertilizingratewassignificantlyhigherthanthatofthispatient(100％VS21．1％．P<0．O1)．Butthecleavageratehasnostatist

7、icaldiference(100％VS100％．P>0．05)．Thispatienthadnormalchromosome(46，XY)andtherewasnodeletioninYchromosome．Hiswifebecamepregnantafteracceptingtwothawedembryosandthengavebirthtoaboywhosechromosomewasnormal(46，XY)．Theaceeptoralsogavebirthtoahealthyboyafterac