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1、作物学报ACTAAGRONOMICASINICA2011,37(12):21232129http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2011.02123玉米抗粗缩病自交系种质的发掘和遗传多样性及其在育种中的应用122212,*薛林张丹徐亮金萌萌彭长俊徐辰武12江苏沿江地区农业科学研究所,江苏如皋226541;扬州大学作物遗传生理省级重点实验室,江苏扬州225009摘要:玉米粗缩病是近十年来危害我国部分玉米产区
2、的主要病害之一,挖掘抗粗缩病玉米种质,进而培育抗病品种是防治粗缩病危害最经济有效的途径。选用184份常用玉米自交系(包括111份普通玉米和73份糯玉米)于2009—2010年在玉米粗缩病重发区江苏沿江地区农业科学研究所试验田进行田间自然鉴定;同时利用117对SSR引物对供试的玉米自交系进行多态性检测,按UPGMA方法,利用Powermarker3.0软件对184份自交系进行系统聚类分析。筛选出2份高抗(T877和YJ7)、6份抗病普通自交系和2份糯玉米抗性种质。基于644个SSR多态性位点,结合系谱资料和育种实践,将184份自交系划为9个杂种优势亚群。自交系4S及其衍生系(第VI亚
3、群)和来源于美国杂交种78599的玉米自交系(第VIII亚群)为玉米抗粗缩病育种的重要种质。关键词:玉米自交系;粗缩病;抗性鉴定;遗传多样性MiningandAnalyzingGeneticDiversityforMaizeRoughDwarfDiseaseRe-sistantGerplasmsandItsApplicationinMaizeBreeding122212,*XUELin,ZHANGDan,XULiang,JINMeng-Meng,PENGChang-Jun,andXUChen-Wu12JiangsuYanjiangInstituteofAgriculturalSci
4、ences,Rugao226541,China;JiangsuProvincialKeyLaboratoryofCropGeneticsandPhysiology,YangzhouUniversity,Yangzhou225009,ChinaAbstract:Maizeroughdwarfdiseasegenerallycausedbyriceblack-streakeddwarfvirus(RBSDV)hasbeenoneofthemaindiseasesinpartsofmaizeproductionareainrecent10years.Miningmaizeroughdwa
5、rfvirus(MRDV)resistantgermplasmandthenbreedingresistantvarietyisaneconomicalandeffectiveapproachtocontrolthedisease.Onehundredandeighty-fourinbredlinesincluding111commonmaizeand73waxymaizewerescreenedforreactiontoMRDVunderfieldconditionsduring2009and2010atNantong,Jiangsuprovince,China.Thegenet
6、icdiversityofthetestedmaterialswasinvestigatedbasedon117poly-morphicSSRmarkers.Clusteranalysisof184inbredlineswasconductedbasedongeneticsimilaritiesfromSSRdatabyusingPowermarker3.0softwarewithUPGMAmethod.Thediseaseevaluationresultedinidentificationoftwoandeightinbredlineswithhighresistanceandr
7、esistancetoMRDV,respectively,amongthemtwowaxyinbredlineswereidentified.UPGMAanalysisindicatedthat184inbredlinescouldbeclassifiedintoninesub-groups,whichweregenerallyconsistentwiththeirknownpedi-greeinformationandbreeder’sexperiences.Two