Hoagland 营养液配方(试验总结).doc

《Hoagland 营养液配方(试验总结).doc》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、Hoagland营养液配方，荷格伦特营养液的配方如下:1.大量元素每升培养液中加入克数或摩尔数盐类浓度g/Lmol/Lg/15LCa(NO3)2·4H2O1.180.00517.7KNO30.510.0057.65MgSO4·7H2O0.490.0027.35KH2PO40.140.0012.12.微量元素每升培养液中加入的毫克数盐类浓度（mg/L）mg/15LH3BO32.8642.9MnCl2·4H2O1.8127.15ZnSO4·7H2O0.223.3CuSO4·5H2O0.081.2H2MoO4·H2O/Na2MoO4·2H2O0.02/0.

2、030.483.每升培养液中加入2mLFeEDTA溶液(即乙二胺四乙酸铁盐溶液)。铁盐溶液：七水硫酸亚铁2.78g乙二胺四乙酸二钠（EDTA-Na2）3.73g蒸馏水500mL配制500mL的铁盐溶液：1.取两只洁净干燥烧杯，分别加入3.73gEDTA-Na2和2.78gFeSO4·7H2O固体。2.取1L纯水煮沸，冷却至70℃后，量取300mL，加入装有EDTA-Na2的烧杯中，进行搅拌溶解。3.量取200mL纯水，冷却后加入另一烧杯中，搅拌溶解。4.然后将FeSO4·7H2O溶液缓缓加入到EDTA-Na2溶液中，边加边搅拌。5.最后，将铁盐溶液倒

3、入棕色瓶中（黑暗），低温保存。通常应当先配出各种盐类的浓缩液。注意避免浓缩液中出现沉淀。使用时按一定的比例加水稀释到要求的浓度。采用循环供液时，营养液中的矿质元素被植物体吸收后，应当及时进行调节，使营养液仍旧符合原配方的要求。以下是老外的有些看不懂呵呵RecipeforHoagland'sCompleteNutrientSolutionThisismadeessentiallyaccordingtothefollowingreference:D.R.HoaglandandD.I.Arnon.Thewater-culturemethodofgrowin

4、gplantswithoutsoil.Calif.Agr.Expt.Sta.Circ.347.1950.Thereisonechangeandthatisintheformofironadded.Preparethefollowingstocksolutions(1-6)andusetheamountsindicatedtoprepare1liter(finalvolume)ofnutrientsolution:1.1.00MNH4H2PO4use1mL/Lofnutrientsolution2.1.00MKNO3use6mL/Lofnutrient

5、solution3.1.00MCa(NO3)2use4mL/Lofnutrientsolution4.1.00MMgSO4use2mL/LofnutrientsolutionMicronutrientstocks:combinethefollowingamountofsaltsinatotalvolumeofoneliterofwater,andthenuse1mL/Lofthisentirestockmixture(5)alongwiththestocksabove(1-4)andtheironstock(6)describedbelowtomak

6、eupatotalof1Lofnutrientsolution.5.2.86gmH3BO31.81gmMnCl2.4H2O0.22gmZnSO4.7H2O0.08gmCuSO4.5H2O0.02gmH2MoO4.H2O(Assaying85%MoO3)6.Ironstock:totheabove5stocksadd0.25mlofthisironstockfor1literofnutrientsolution.Tomakeuptheironstock,take26.1gEDTAanddissolvein286mlwaterthathas~19gKOH

7、.Thendissolve24.9FeSO4.7H2Oin~500mlwater.SlowlyaddtheironsulfatesolutiontothepotassiumEDTAsolutionandaeratethissolutionovernightwithstirring.ThepHrisestoabout7.1andthesolutioniswineredandverylittleprecipitationoccurs.Maketo1literfinalvolumeandstoreinabottlecoveredwithfoil(dark)

8、.Note:Hoagland'srecipecalledfor1mlof0.5%irontartratest