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1、2017,36(6):1102-1110农业环境科学学报2017年6月JournalofAgro-EnvironmentScience黄献培,向垒,尹倩,等.环丙沙星高低累积菜心根际土壤酶活性和微生物学特性差异研究[J].农业环境科学学报,2017,36(6):1102-1110.HUANGXian-pei,XIANGLei,YINQian,etal.Variationsinrhizosphereenzymaticactivitiesandmicrobiologicalcharacteristicsbetweenhigh
2、andlowciprofloxacinaccumulationcultivarsofBrassicacampestrisL.[J].JournalofAgro-EnvironmentScience,2017,36(6):1102-1110.环丙沙星高低累积菜心根际土壤酶活性和微生物学特性差异研究*黄献培,向垒,尹倩,赵海明,喻乐意,李彦文,蔡全英,莫测辉(暨南大学环境学院,广东省环境污染控制与修复材料工程技术研究中心,广州510632)摘要:为查明环丙沙星高、低累积菜心根际消除环丙沙星(CIP)高低差异的微生物学机理,通
3、过室内土壤盆栽实验,研究了这两种菜心根际土壤中酶活性和微生物学特征差异。结果表明:种植高、低累积菜心均促进土壤CIP的消除,在高污染水平时,高累积菜心对土壤CIP的消除效果显著高于低累积菜心,其降解率分别为48.7%和39.4%(P<0.05);高累积菜心根际土壤的可培养细菌数量和细菌群落结构多样性与丰富度(尤其含CIP特异降解菌Betaproteobacteria的相对丰度),以及过氧化氢酶和脲酶活性均显著高于低累积菜心。研究结果初步揭示了高累积菜心根际去除CIP能力更强且体内吸收累积CIP更多这一初步机理。关键词:菜
4、心;环丙沙星;吸收累积;品种差异;根际微生物;酶活性中图分类号:X171.5文献标志码:A文章编号:1672-2043(2017)06-1102-09doi:10.11654/jaes.2017-0557VariationsinrhizosphereenzymaticactivitiesandmicrobiologicalcharacteristicsbetweenhighandlowciprofloxacinaccumulationcultivarsofBrassicacampestrisL.HUANGXian-pei
5、,XIANGLei,YINQian,ZHAOHai-ming,YULe-yi,LIYan-wen,CAIQuan-ying,MOCe-hui*(GuangdongProvincialResearchCenterforEnvironmentPollutionControlandRemediationMaterials,SchoolofEnvironment,JinanUni-versity,Guangzhou510632,China)Abstract:Therhizospheremicroecologyplaysakeyr
6、oleinuptakeandaccumulationofpollutantsinplants.Thevariationsinrhizosphereenzymaticactivitiesandmicrobiologicalcharacteristicsbetweenhighandlowciprofloxacin(CIP)accumulationcultivarsofBrassicacampestrisL.wereinvestigatedusingpotexperimentstoexpoundthemicrobiologic
7、almechanismofvariationsinremovalofCIPintherhi-zosphereofthetwocultivars.ResultsshowedthatcultivationofboththecultivarssignificantlyenhancedthedegradationofCIPinsoil.AthighCIPlevel,theremovalefficiencyofCIPintherhizosphereofthehighaccumulator(48.7%)wassignificantl
8、yhigherthanthatofthelowaccumulator(39.4%)(P<0.05).Theamountofcultivablebacteria;therichnessandevennessofthesoilbacterialcommunity,especiallytheBetaproteobacter