资源描述：
《尼古丁论文：尼古丁降解菌的筛选鉴定及降解特性的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、尼古丁论文：尼古丁降解菌的筛选鉴定及降解特性的研究【中文摘要】本文利用富集筛选的方法从杭州农药厂的活性污泥中分离得到两株尼古丁高效降解菌,分别命名为HZN1和TW5,经过对这两株菌的生理生化特性、形态特征和16SrDNA序列同源性分析,将其分别鉴定为申氏杆菌属(Shinellasp.)和假单胞属(Pseudomonassp.)。研究表明,菌株HZN1和TW5都能以尼古丁为唯一碳源、氮源和能源生长,能在质量浓度为4.0g/L的尼古丁基础盐培养基中生长,菌株生长和降解尼古丁的最适温度均为30℃,最适pH值为6.5~7.5。菌株HZN1分别能在4h、9h、12h内
2、将初始浓度分别为0.2、0.5、1.0g/L的尼古丁降解接近完全,而外加碳源葡萄糖会减缓HZN1降解尼古丁的速率;温度和pH值对HZN1的降解效率影响不大;菌株TW5分别能在6h和9h内将初始浓度分别为0.2和0.5g/L的尼古丁降解接近完全,当尼古丁质量浓度上升为1.5g/L时,降解效率受到明显影响,12h内只能降解70%的尼古丁,温度和pH值对TW5的降解效率影响较大。进一步利用气质联用仪(GC-MS)研究了菌株HZN1降解尼古丁的代谢产物,根据检测到的三个产物:麦思明、可铁林和尼古提林,推测了菌株HZN1在降解尼古丁过程中可能存在的降解途径。菌株HZN
3、1和TW5具有在恶劣环境中处理高浓度尼古丁废水的潜力,其中HZN1所属的申氏杆菌属(Shinellasp.)还未见报道有能够降解尼古丁的菌种,我们的研究为生物降解尼古丁提供了新的依据。【英文摘要】Thenicotine-degradingbacteriumHZN1andTW5wereisolatedfromactivatedsludgeofHangzhoupesticidefactoryandidentifiedasShinellasp.andPseudomonassp.basedontheirphysiologicalcharacteristicsanda
4、nalysisof16SrDNAgene,respectively.StrainHZN1andTW5arecapableofusingnicotineasthesolesourceofcarbon,nitrogenandenergy,andcangrowthonthemineralsaltsmediumwithnicotineconcentration4.0g/L.TheoptimumtemperatureandpHforstrainHZN1andTW5growthandnicotinedegradationwere30℃and7.0,respectivel
5、y.StrainHZN1coulddegradeapproximately100%of0.2,0.51.0g/Lofnicotinewithin4,9,12h,respectively;thedegradationofnicotinewasslightlyslowerwiththepresenceofglucose;strainHZN1coulddegradenicotineefficientlyinarelativelybroadrangeoftemperature(23,30,37℃)andpHvalues(5.5~9.0).StrainTW5could
6、degradeapproximately100%of0.2,0.5g/Lofnicotinewithin6,9h,respectively;itcouldonlydegrade70%of1.5g/Lofnicotineat12h;temperatureandpHhavehigheffectsonnicotinedegradationbystrainTW5.ThreeintermediatemetaboliteswereproducedbythestainHZN1andidentifiedascotinine,myosmineandnicotyrineusin
7、ggaschromatography-massspectrometry(GC-MS).Andpossiblenicotine-degradingpathwayofstrainHZN1waspresumed.TheseresultsindicatethatthestrainHZN1andTW5couldpotentiallybeusefulfortheremediationofnicotinepollutioninmal-condition.Thisisthefirstreportofnicotine-degradingstrainfromthegenusof
8、Shinella.Ourfindingswouldp
