宫粉羊蹄甲花粉离体萌发培养基的筛选.pdf

《宫粉羊蹄甲花粉离体萌发培养基的筛选.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、南方农业学报JournalofSouthernAgriculture2015，46(12)：2173—2179ISSN2095—1191；CODENNNXAABhttp：／／www．11f：nyxb．cornDOI：10．3969，j：issn．2095-1191．2015．12．2173宫粉羊蹄甲花粉离体萌发培养基的筛选贺庆梅，陈岩岩，李启虔，黄承潇，罩勇荣‘(河池学院化学与生物工程学院，广西宜州546300)摘要：【目的】筛选最适宫粉羊蹄甲花粉离体萌发的培养基及培养条件，为其花粉活力检测、品种繁育及苗木生产提供参考依据。【方法】宫粉羊蹄甲花粉粒采用液态培

2、养法，设置培养基组分、pH和温度等8个影响因子的梯度值进行逐步迭代优化，以缺素培养为对照(CK)，探讨其花粉萌发效果。【结果】培养基各组分对宫粉羊蹄甲花粉离体萌发的影响效果迥异，其中H3BO，对花粉萌发起决定性作用，花粉萌发对蔗糖较敏感。宫粉羊蹄甲花粉在添加8．0％蔗糖、10．0％聚乙二醇．6000(PEG．6000)、0．0020％H墨03、0．02％CaCl2·2H20、0．02％MgS04·7H20及0．010％KNO，的液体培养基中，于pH6．8、恒温29℃条件下培养约20min可观察到最佳萌发效果，萌发率均值达91．230％。【结论】宫粉羊蹄甲花粉

3、在液体培养基各组分及培养条件最佳组合中离体培养才能提高萌发率。花粉管才能正常生长。关键词：宫粉羊蹄甲；花粉；离体萌发；培养基；筛选中图分类号：$687．1文献标志码：A文章编号：2095—1191(2015)12—2173—07ScreeningofculturemediumforjnvitropollengerminationofBauhiniavariegateL．HEQing-mei，CHENYan—yan，LIQi-qian，HUANGCheng—xiao，QINYong-rong+(CollegeofChemicalandBiologicaleng

4、ineering，HeehiUniversity，Yizhou，Guangxi546300，China)Abstract：【Objective】TheoptimalmediumandcultureconditionforinvitropollengerminationofBauhiniavariegateL．werescreened，inordertoprovidereferencebasisforpollenviabilitydetermination，varietiesbreedingandnurserystockproduction．【Method]T

5、hepollengrainswerecultivatedwithliquidmedium．And8factorsaffectingpoHengermina—tionwereselected，includingmediumcomposition，pHandtemperatureetc．Andthegradientvaluesof8factorsweresetbyiterativeoptimizationmethod．Thentheinvitropollengerminationeffectwascomparedwithgerminationeffectofpo

6、lleninmediumlackingnutritionalelements(ascontr01)．【Result】TheresultsshowedthattherewerelargedifferencesbetweeneffectsofmediumcompositiononinvitropollengerminationofB．variegateL。Mostofall，H3803playedthedecisiveroleinpollengermination，andthegerminatingpollenwasmoresensitivetosucYose．

7、WhenB．variegateL．invitropollenwereculturedintheliquidmediumsupplementedwith8．0％sucrose，10．0％PEG一6000(polyethyleneglycol一6000)，0．0020％H3803，0．02％CaCl2·2H20，0．02％MgS04·7H20and0．010％KN030．02％at29℃andpH6．8for20min，theoptimalgerminationeffectWasobtained，theaveragegerminationratereachedu

8、pto91．230％．【Conclusion】The