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ID:49253591
大小:1.89 MB
页数:66页
时间:2020-02-02
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1、TranscriptanalysisandreconstructionBrazil2001GenesWhyarethereonlyafewtensofthousandsofgenesinthehumangenome?Howdogenesexpressthemselvestomanufacturetheproteome?Howcanavailablesequenceinformationbeprocessedinordertodeliverunderstandingofgeneexpression?Genomicex
2、pressionWithineukaryotes,geneshavesharedbasiccharacteristics.Theyhavesingleormultipleexonsandintronsdistributedalongthegeneincodingandnon-codingregionswith5’FlankingregionwithtranscriptionregulationsignalsTranscriptioninitiationstartsite(5’)Initiationcodonforp
3、roteincodingsequenceExon-intronboundarieswithsplicesitesignalsattheboundariesTerminationcodonforproteincodingsequence3’signalsforregulationandpolyadenylationGCboxCAATTATAGCboxTranscriptionInitiationSiteExon1InitiationCodonGTTranscriptionInitiationSiteAGGTIntro
4、n1Intron2Exon2GCboxGCboxCAATTATATranscriptionInitiationExon1InitiationCodonGTAGGTAGIntron1Intron2Exon2Exon3StopCodonAATAAPoly(A)additionsite5’FlankingregionPre-mRNAMaturemRNAGeneExpressionTranscriptionproductscanvary.Transcriptioninitiationatthestartsite(TSS)E
5、xonlengthExonprescence/absenceinthematuretranscriptAlternatetranscriptionterminationandpolyadenylationAlternativedonorandacceptorsplicesitesAlternativepolyadenylationExonskippingExamplesofalternativesplicingGCboxGCboxCAATTATATranscriptionInitiationExon1Initiat
6、ionCodonGTAGIntron1Exon3StopCodonAATAAPoly(A)additionsite(s)Exon2SKIP3’FlankingregionPre-mRNAMaturemRNACapturingexpressedtranscriptsDatabases-SequencesdbESTSeveralcollapseddatasetsTIGR-THCAllgenesUnigeneBodyMapSTACKSeveralmorespecialisedGenomeSequenceasitappea
7、rs:ExpressionCaptureSerialAnalysisofGeneExpressionDNAfragmentsthatactasuniquemarkersofgenetranscripts.AssayofnumbersofeachmarkerinasetofsequenceyieldsameasureofgeneexpressionArrayLaydownofsequenceclonestoprovideanorganisedseriesforhybridisationResolutionofCapt
8、uredExpressionESTSLowresolution,broadcapture,providestemplateforSAGEandArraySAGEMediumresolution,needtemplate,noisecanbeanissue,stoichiometryisrevealedbutstandardisationaproblemARR
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