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ID:49005376
大小:1.39 MB
页数:34页
时间:2020-01-29
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1、AntibodiesSecretedbyBlymphocytesGreatdiversityandspecificity:>109differentantibodies;candistinguishbetweenverysimilarmoleculesTagparticlesforclearance/destructionProtectagainstre-infection(vaccines)AntibodyStructureIgdomain:110aminoacids;globulardomai
2、nusedinmanyproteins;Variabledomains,Constantdomains,HingeFab:fragmentantigenbindingFc:fragmentcrystallizable(effectorfunctions)©NewSciencePressLtd.2003TheImmunoglobulinSuperfamily(afewexamples)VariabilityinantibodiesisclusteredintheloopsintheVariabled
3、omainsoftheheavyandlightchains(green);theseregionsareresponsibleforbindingtoantigenAntibodyClasses:Structure©NewSciencePressLtd.2003AffinityandAvidityAffinity:thestrengthofbindingbetweenasinglebindingsiteandasingleligand[A][B][AB]Avidity:thestrengthof
4、bindingbetweenamoleculeandacomplexligand,e.g.iftherearemultiplebindingsitesthentheaviditymaybeincreasedbyincreasingthenumberofbindingsitesorbyincreasingtheaffinityofthosebindingsitesKD=AffinityandAvidityIIIgMisproducedearlyinanimmuneresponsewhentheaff
5、inityforantigenoftenislow;asanimmuneresponsecontinues,antibodyaffinityisimproved,thisiscombinedby“classswitching”totheuseofsmallermolecules(IgG,IgEandIgA).Theincreasedaffinitycompensatesforthedecreaseinnumberofbindingsitesinmaintainingtheoverallavidit
6、yforantigen©NewSciencePressLtd.2003MonoclonalAntibodiesSingleantibody(allsameHandLchains)MadebyfusionofBcellstoatransformedcelllineoftheplasmacelltypeandselectionfor“hybridomas”thatproduceantibodywiththedesiredpropertiesStandardized,unlimitedreagentfo
7、rdiagnosisortherapy(humanantibodiesor“humanized”antibodiescanbemade)Polyclonalvs.MonoclonalAntibodiesImmunizeIndividualSerumPolyclonalantibodies7+daysPurifyantibodiesBlymphocytes3daysImmortalize“hybridoma”cDNAcloningMonoclonalAntibodiesGenerationofMon
8、oclonalAntibodies©NewSciencePressLtd.2003MonoclonalantibodiesusedinmedicineStandardized,unlimitedreagentsfordiagnosisortherapy(humanantibodiesor“humanized”antibodiescanbemade)©NewSciencePressLtd.2003GenerationofantibodydiversityHowdowemake>109
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