龙蒿生殖生物学及繁殖方法研究

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1、新疆农业人学WrWitt鳩生殖生物学及繁殖方法的研究姓名：原丽华申请学位级别—硕士指导教师：秦勇20040601中文摘要通过实地观察右蜡切片和组织培养等方法对新疆龙蒿的生物学特性营养繁殖有性生殖大小砲子及雌雄配子体的发育等生殖生物学过程进行了研究并对其全后的开发提出了一些建议研究结果简要介绍如下1、繁殖方式兼具有性的种子繁殖和以根茎进行的营养繁殖两种类型两种繁殖方式均较好，，2龙蒿种子千粒重0.0605g,属小粒种予直接播种不易出苗根茎繁殖是一种简单的繁殖方法基质和激素浓度对龙蒿打•插成活有较大影响在所选用的四种基质中蛭石+鸡粪+炉灰渣效果最好IBA的浓度为50ppm时生根作用显著、组织培

2、养可以快速获得整齐卞致的幼苗、BAO.1-0.3mg/L+IBA0.02-0.06mg/L增殖效果明显IBA在；0.1-2wg/E范围内较NAA的生根效果好3200250ShgL-.GAs5%2§%PEG0.5%NaHCO3溶液处理可显著地提高龙蒿种韦的各项发芽指标2528可以明越提庵龙蒿种子的各项发芽指标但超过30则会明显抑制其萌发3h以内的浸种利于其萌发但浸种时间不宜超过6h光照可以显著促进龙蒿种子的萌发,5低温处理吋间在不超过72h的情况下不会影响龙蒿利;子的萌发，4有性生殖时雄蕊的花药壁发育为双了叶植物型绒毡层为腺质绒毡层小抱子母细胞减数分裂为连绥型小何子四分体为四面体型和庁右对称

3、型成熟花粉粒为三細胞花粉舌状花雌蕊的子房下位二心皮一室胚珠一枚基生胎座倒生胚珠单珠被薄珠心大泡子四分体为直线形合点端大砲子为功能大他了胚囊发育类型为蓼型StudyontheReproductiveBiologyandPropagataingmethodsofArtemisiadracunculusL・YuanLihua(Vegetablebreeding)SupervisedbyQinYongAbstractWiththeaidofparaffintechniques,thebiologicalcharacters,thegrowthanddevelopmentoftheshoots9me

4、gasporogenesis,microspogenesisanddevelopmentofgametophytesandembryogenywerestudied,themainresultsinthisreportswereasfollowing:1Thisspeciesprocessedbothsexualreproductionbyseedsandvegetativepfopagationbyrootcrown,populationregenerationwasgood・2TheseedsofArtemisiadracunculuswereeasytogerminate.Cutti

5、ngroot-stempropagatingmethodwassimplebutpropagatingratewaslow.3Duringthesexualreproduction,thedevelopmentoftheantherwallfollowedthedicotyledontype・Tapetumwasthesecretorytype.Thecytokinesisofthemicrosporemothercellwasofthesuccessivetype.Themicrosporetetradsweretrahedral.Thematurepollengrainwastheth

6、ree-celledtype.Theovarywasepigynous,bicarpellaryandtetraloules.Eachloculecontainedasingleovule.Thematureovuleswereamphitropous,untegmic,tenuinucellateandhadhypostase・Themagsporetetradswerelinear.Usually.thechalazalmegasporewasfunctional.Thedevelopmentofthefemalegameto-phyewasofthepolygonumtype.4GA

7、3NaHCO3PEGcouldincreasethegerminationindexesofArtemisia〜°Cdracimculusseeds,withconcrentrationof200250mg/L,525%and0.5%;Increasingtemperaturecouldprompotallgerminationindexesand2528wasthemostsuitabletemperature;sho