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High-Affinity Manganese Uptake by the Metal Transporter NRAMP1 Is Essential for Arabidopsis Growth in Low Manganese Conditions

High-Affinity Manganese Uptake by the Metal Transporter NRAMP1 Is Essential for Arabidopsis Growth in Low Manganese Conditions

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时间：2019-08-06

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1、ThePlantCell,Vol.22:904917,March2010,www.plantcell.orgã2010AmericanSocietyofPlantBiologistsHigh-AffinityManganeseUptakebytheMetalTransporterNRAMP1IsEssentialforArabidopsisGrowthinLowManganeseConditionsWRe´myCailliatte,AdamSchikora,1Jean-Franc¸oisBriat,Ste´

2、phaneMari,andCatherineCurie2LaboratoiredeBiochimieetPhysiologieMole´culairedesPlantes,CentreNationaldelaRechercheScientifique,Unite´MixtedeRecherche5004,InstitutdeBiologieInte´grativedesPlantes,F-34730Montpelliercedex2,FranceIncontrastwithmanyotheressentia

3、lmetals,themechanismsofMnacquisitioninhighereukaryotesareseldomstudiedandpoorlyunderstood.WeshowherethatArabidopsisthalianareliesonahigh-affinityuptakesystemtoacquireMnfromthesoilinconditionsoflowMnavailabilityandthatthisactivityiscatalyzedbythedivalentmet

4、altransporterNRAMP1(forNaturalResistanceAssociatedMacrophageProtein1).Thenramp1-1loss-of-functionmutantgrowspoorly,containslessMnthanthewildtype,andfailstotakeupMninconditionsofMnlimitation,thusdemonstratingthatNRAMP1isthemajorhigh-affinityMntransporterinA

5、rabidopsis.BasedonconfocalmicroscopyobservationofanNRAMP1-greenfluorescentproteinfusion,weestablishedthatNRAMP1islocalizedtotheplasmamembrane.ConsistentwithitsfunctioninMnacquisitionfromthesoil,NRAMP1expressionisrestrictedtotherootandstimulatedbyMndeficienc

6、y.Finally,weshowthatNRAMP1restoresthecapacityoftheiron-regulatedtransporter1mutanttotakeupironandcobalt,indicatingthatNRAMP1hasabroadselectivityinvivo.TheroleoftransportersoftheNRAMPfamilyiswellestablishedinhighereukaryotesforironbuthasbeencontroversialfo

7、rMn.ThisstudydemonstratesthatNRAMP1isaphysiologicalmanganesetransporterinArabidopsis.INTRODUCTIONbychronicinhalationofMnoxidesandresultsinneurologicaldamages(Keenetal.,2000).Inplants,highconcentrationsofMnAllorganismsrequiretracelevelsofMnforsurvival.Mnis

8、acausechlorosis,brownspecklesonmatureleaves,andnecrosis,transitionmetalthatcanexistinseveraldifferentvalencestateswhichresultinreducedcropyield(Marschner,1995).andthereforeplaystheroleofcatalystinelectrontransferMnd

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