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1、FoodChemistry134(2012)387–391ContentslistsavailableatSciVerseScienceDirectFoodChemistryjournalhomepage:www.elsevier.com/locate/foodchemShortcommunicationMetaproteomiccharacterisationofaShaoxingricewine‘‘wheatQu’’extracta,baacaa,b,⇑BoZhang,Ling-QiongKong,YuCao,Guang-FaXie,Zh
2、eng-BingGuan,JianLuaSchoolofBiotechnologyandKeyLaboratoryofIndustrialBiotechnology,MinistryofEducation,JiangnanUniversity,Wuxi,Jiangsu214122,PRChinabNationalEngineeringLaboratoryforCerealFermentationTechnology,JiangnanUniversity,1800LihuRoad,Wuxi,Jiangsu214122,PRChinacKeyLa
3、boratoryofTechnologyandEquipmentforChineseRiceWine,ZhejiangProvince,ChinaShaoxingRiceWineGroupCo.Ltd.,Shaoxing312000,PRChinaarticleinfoabstractArticlehistory:Thestarterculture‘‘wheatQu’’hasbeenusedinthefermentationofChinesericewineforhundredsofReceived9May2011years.Inthisst
4、udy,aShaoxingricewine,‘‘wheatQu’’(machine-madetype),extractwasanalysedbyReceivedinrevisedform6January2012usingthemetaproteomicapproach.Allvisible145separateproteinspotsinthetwo-dimensionalelectro-Accepted9February2012phoresisgelwereexcisedandidentifiedbyMatrix-AssistedLaserD
5、esorptionIonisation-TimeofFlight/Availableonline17February2012TimeofFlightMassSpectrometry(MALDI-TOF/TOFMS)analysis.Elevenspotsrepresent10proteinsthatoriginatefromthesecretionoffungiorbacteria,112spotsrepresent43proteinsfromplants,andoneKeywords:spotrepresentsproteinfromfau
6、na.Theseproteinspossessdiversefunctionsandparticipateinthefer-ChinesericewinementationprocessofShaoxingricewine.ThisisthefirstreportedstudyontheecosystemofatraditionalShaoxingStarterculturewinestarterculture,usinganovelmethodthatistermed‘‘metaproteomics’’.MetaproteomicsÓ2012
7、ElsevierLtd.Allrightsreserved.MS1.IntroductionVariousmicroorganisms,includingfungiandbacteria,canbegrownin‘‘wheatQu’’becauseitispreparedfromunsterilisedandChinesericewinehasa2000year-longhistoryandisoneoftheunheatedwheat.Theinteractionsofsymbiosisandmetabiosis,oldestdrinksi
8、ntheworld.ItisapopularalcoholicbeverageinChi-amongthedifferentmicroorganisms,forma