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ID:39970632
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页数:5页
时间:2019-07-16
《hydrogel microchannel for cell adhension》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、ViewOnline/JournalHomepageLabonaChipDynamicArticleLinksCitethis:DOI:10.1039/c2lc40611jwww.rsc.org/locTECHNICALINNOVATIONCircular,nanostructuredandbiofunctionalizedhydrogelmicrochannelsfordynamiccelladhesionstudies{abccabSebastianKruss,*LuiseErpenbeck,MichaelP.Scho¨nandJoachimP.Spa
2、tzReceived28thMay2012,Accepted13thJuly2012DOI:10.1039/c2lc40611jWereportonamethodtofabricatebiofunctionalizedpolyethyleneglycolhydrogelmicrochannelswithadjustablecircularcross-sections.TheinnerchannelsurfacesaredecoratedwithAu-nanoparticlearraysoftunabledensity.TheseAu-nanoparticl
3、esarefunctionalizedwithbiomoleculeswhereasthehydrogelmaterialprovidesaninertandbiocompatiblebackground.Thistechnologyprovidescontroloverflowconditions,channelcurvatureandbiomoleculedensityonthechannelsurface.Itcanbeappliedforbiophysicalstudiesofcell-surfaceinteractionsmimicking,fo
4、rexample,leukocyteinteractionswiththeendotheliallininginsmallvessels.10Introductionmajorchallenge.Especiallyforbiologicalapplications,cova-lentandsite-directedimmobilizationofbiomoleculesisdesir-Overthepastdecade,microfluidicshasbecomeavaluabletoolforable.Closelyrelatedtosurfacefu
5、nctionalizationisthequestioncellbiologybecauseitcanprovidecontroloversmallfluidofhowmanymoleculesasurfacemustpresenttomimica1volumesandbiophysicalparameterssuchasshearstress.Sofar,biologicalinterfaceorperformaspecifictask.Thedensityandmostoftheapproachesusedtofabricatemicrostructu
6、redsystemsspatialpresentationofadhesionsitesonsurfacescanhavea2forcelladhesionstudiesarebasedonsoftlithography.Withtop-dramaticinfluenceoncelladhesion.11Asaprominentexample,downmethods,thecross-sectionisusuallylimitedtoarectangularadhesiveinteractionsofcirculatingbloodcellswiththe
7、geometry.However,thetopographyandthree-dimensionalshapeendotheliumduringinflammationaregovernedbytheup-andDownloadedbyColumbiaUniversityon06August2012ofthesubstratemustbetakenintoaccountwheninvestigatingdownregulationofcellsurfacereceptordensity.12cellularinteractionsascellshavebe
8、enshowntorespondtotheTheuseofPDMS
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