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1、胎鼠胃上皮细胞增殖、凋亡与胃形态发生的关系作者：林雪梅,汪维伟,李芳菲单位：重庆医科大学基础医学院组织学与胚胎学教研室,基础医学研究所,重庆市生物化学与分子药理学重点实验室,重庆提要:目的研究胎鼠胃上皮细胞增殖、凋亡的时空规律及其与胃形态发生的关系。方法每间隔0.5d取E11~15.5d小鼠整胎,横断连续切片,观察胃形态发生,体视学法测量胃贲门、幽门、大弯、小弯上皮分裂细胞密度(DMC)、凋亡小体密度(DAB)。结果①E11.5d胃开始发育,E12d胃底膨出,胃大、小弯出现。②上皮细胞DMC峰值均在DAB峰值之前。③贲门处
2、DMC无明显峰值,DAB峰值在E11.5、12.5、13.5d;幽门处DMC峰值在E12.5d,DAB峰值在E14d;大弯DMC峰值在E11.5、12d时,DAB值持续较低;小弯DMC峰值在E11.5d,DAB峰值在E12.5d。结论胎鼠胃上皮细胞的增殖及凋亡与胃发生有密切的时空关系。关键词:胎鼠;胃;增殖;凋亡;形态发生中图法分类号:Q954.48;Q954.58文献标识码:ACorrelationofgastricmorphogenesiswithproliferationandapoptosisofgastricep
3、i-theliuminembryonicmiceLINXue-mei,WANGWei-wei,LIFang-fei(DepartmentofHistologyandEmbryology,ChongqingMedicalUniversity,Chongqing400016,China)Abstract:ObjectiveTostudythespatiotemporaldistributionsofepithelialproliferationandapoptosis,andthecorrelationofmorphogene
4、siswiththemindevelopingstomach.MethodsSerialsectionsoffetalmiceaged11-15.5daysweremade.Themorphogenesisofstomachwasobservedunderlightmicroscope.Theden-sityofmitoticcells(DMC)andapoptoticbodies(DAB)ofepitheliuminthedevelopingcardia,pylorus,grea-tercurvature,lesserc
5、urvatureweremeasuredwithstereologicalmethod.ResultsThemorphologicaldevelop-mentofstomachtookplaceonthe11thdayofmouseembryodevelopmen.tThefundus,greatercurvatureandlessercurvaturewereobservedonthe12thday.ThepeakvalueofDMCallappearedbeforethepeakvalueofDAB.Intheepit
6、heliumofcardia,noobviouspeakvalueofDMCwasobservedandthepeakvalueofDABwasonthe11.5th,12.5th,13.5thday.Intheepitheliumofpylorus,thepeakvalueofDMCwasonthe12•5thday,thepeakvalueofDABwasonthe14thday.Intheepitheliumofgreatercurvature,thepeakvalueofDMCwasonthe11.5th
7、and12thday,theDABvaluewaslow.Intheepitheliumoflessercurvature,thepeakvalueofDMCwasonthe11.5thday,thepeakvalueofDABwasonthe12.5thday.ConclusionTherewasveryclosespatiotemporalrelationshipbetweenthecellproliferation,cellapoptosisandthemorphologicaldevelopmentofstomac
8、h.Keywords:fetalmouse;stomach;proliferation;apoptosis;morphogenesis由于涉及器官众多,又受复杂外环境的影响,长久以来,胚胎消化管的发生是一研究难点,迄今机制尚不清楚[1,2]。既往研究较多的是小肠,一般认为与其上皮细胞增殖及凋亡、肠壁平滑
