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时间:2019-03-06
《几种水蛭抗凝血物质提取及活性分析new》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、万方数据第5l卷第4期中山大学学报(自然科学版)2012年7月ACTASCIENTIARUMNATURALIUMUNIVERSITATISSUNYATSENIVoL51No.4JIlL2012几种水蛭抗凝血物质提取及活性分析+张彬1,汪波2,龚元3,于翔4,吕军仪’(1.广西水产研究所广西水产遗传育种与健康养殖重点实验室,广西南宁530021;2.北京师范大学珠海分校,广东珠海519085;3.荆州市民康生物科技有限公司,湖北荆州434300;4.辽宁省淡水水产科学研究院。辽宁迂阳111000;5.中山大学生命科学学院,广东广州510275)摘要:研究
2、3种提取方法对菲牛蛭不同处理品的抗凝作用,采用筛选的最佳提取方法分析了5种水蛭各处理品的抗凝血酶活性,并比较了3个不同地理分布野生菲牛蛭和2个不同饵料饲喂菲牛蛭各处理品的抗凝血酶活性。结果表明:丙酮浸提法在菲牛蛭各处理品中均具有最高的提取得率,所测抗凝血酶活性显著高于其他方法(P3、有较强的体外抗凝血酶活性,关键词:水蛭;菲牛蛭;凝皿酶;抗凝作用;提取液干体粉末和唾液腺分泌物抗凝活性均无显著差异(P>且人工养殖对菲牛蛭的抗凝活性未产生不利影响。中图分类号:Q553文献标志码:A文章编号:0529—6579(2012)04-0092-05AnticoagulantActiveSubstancesExtractionandAnti-ThrombinActivityAnalysisofSeveralSpeciesofLeechesZHANGBinl。WANGB02,GONGYuan3,ⅢXian94,LV胁矿(1.GuangxiKeyL4、aboratoryofAquaticGeneticBreedingandHealthyAquaculture,GuangxiInstituteofFisheries,Nanning53002l,China;2.BeijingNormalUniversity。ZhuhaiCampus,Zhuhai519085,China;3.JingzhouMinKangBiotechnologyCompanyLimited,Jingzhou434300,China;4.FreshwaterFisheriesResearchInstituteofLiaoningProv5、ince,LiaoyangI11000,China;5.SchoolofLifeSciences。SunYat—senUniversity,Guangzhou510275,China)Abstract:Theanticoagulantactivityinvitroofthreedifferentsampleisolatedfromtheleech,Hirudinar-iaman///ens/swiththleedifferentextractingmethodsWaSstudied,andutilizedtheoptimalextractingproc6、ess,theanti.thrombinbioactivityoffivespeciesofleecheswereanalyzed,andcomparedtheanti—thrombinbioactivityofthreespeciesofwildH.manillensisfromdifferentgeographicaldistributionandtwospeciesofeuhuredH.man///ens/srearedwitlIdifferentdiets.Theresultsshowedthatamongthem,theextractspro7、ducedbyacetoneprecipitationhadthehighestyield,andalsodisplayedthestrongeranti—coagulantactivitytestedthanothers(P<0.05).Thetestdisplayedthreespeciesofnon-bloodsuckingleeches(吼厶砌施p咖,觋血眦砌acranuiataand既以舢砌/aev/s)hadnoorloweranti-thrombinactivity,andthetwospeciesofblood—suckingleech8、es(日.man///ens/sandH/rudo,咖D,妇)hadhigher·收稿日期:2
3、有较强的体外抗凝血酶活性,关键词:水蛭;菲牛蛭;凝皿酶;抗凝作用;提取液干体粉末和唾液腺分泌物抗凝活性均无显著差异(P>且人工养殖对菲牛蛭的抗凝活性未产生不利影响。中图分类号:Q553文献标志码:A文章编号:0529—6579(2012)04-0092-05AnticoagulantActiveSubstancesExtractionandAnti-ThrombinActivityAnalysisofSeveralSpeciesofLeechesZHANGBinl。WANGB02,GONGYuan3,ⅢXian94,LV胁矿(1.GuangxiKeyL
4、aboratoryofAquaticGeneticBreedingandHealthyAquaculture,GuangxiInstituteofFisheries,Nanning53002l,China;2.BeijingNormalUniversity。ZhuhaiCampus,Zhuhai519085,China;3.JingzhouMinKangBiotechnologyCompanyLimited,Jingzhou434300,China;4.FreshwaterFisheriesResearchInstituteofLiaoningProv
5、ince,LiaoyangI11000,China;5.SchoolofLifeSciences。SunYat—senUniversity,Guangzhou510275,China)Abstract:Theanticoagulantactivityinvitroofthreedifferentsampleisolatedfromtheleech,Hirudinar-iaman///ens/swiththleedifferentextractingmethodsWaSstudied,andutilizedtheoptimalextractingproc
6、ess,theanti.thrombinbioactivityoffivespeciesofleecheswereanalyzed,andcomparedtheanti—thrombinbioactivityofthreespeciesofwildH.manillensisfromdifferentgeographicaldistributionandtwospeciesofeuhuredH.man///ens/srearedwitlIdifferentdiets.Theresultsshowedthatamongthem,theextractspro
7、ducedbyacetoneprecipitationhadthehighestyield,andalsodisplayedthestrongeranti—coagulantactivitytestedthanothers(P<0.05).Thetestdisplayedthreespeciesofnon-bloodsuckingleeches(吼厶砌施p咖,觋血眦砌acranuiataand既以舢砌/aev/s)hadnoorloweranti-thrombinactivity,andthetwospeciesofblood—suckingleech
8、es(日.man///ens/sandH/rudo,咖D,妇)hadhigher·收稿日期:2
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