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1、AGUIDETOPROTEINISOLATIONThispageintentionallyleftblank.
AGUIDETOPROTEINISOLATIONbyCliveDennisonSchoolofMolecularmidCellularBiosciences,UniversityofNatal,Pietermaritzburg.SouthAfricaKLUWERACADEMICPUBLISHERSNEWYORK,BOSTON,DORDREC
2、HT,LONDON,MOSCOWeBookISBN:0-306-46868-9PrintISBN:0-792-35751-5©2002KluwerAcademicPublishersNewYork,Boston,Dordrecht,London,MoscowAllrightsreservedNopartofthiseBookmaybereproducedortransmittedinanyformorbyanymeans,electronic,mechanical,recording,orotherwi
3、se,withoutwrittenconsentfromthePublisherCreatedintheUnitedStatesofAmericaVisitKluwerOnlineat:http://www.kluweronline.comandKluwer'seBookstoreat:http://www.ebooks.kluweronline.comContentsAcknowledgements....................................................
4、....ixPreface............................................................................xiChapter1Anoverviewofproteinisolation..............................11.1WHYDOIT?.....................................................................................
5、11.2PROPERTIESOFPROTEINS................................................................21.3THECONCEPTUALBASISOFPROTEINISOLATION............................31.3.1Wheretostart?.......................................................................41.3.2Wh
6、entostop?.....................................................................51.4THEPURIFICATIONTABLE...............................................................61.5CHAPTER1STUDYQUESTIONS........................................................7Chapte
7、r2Assay,extractionandsub-cellularfractionation....82.1BUFFERS..........................................................................................82.1.1Makingabuffer.......................................................................112.1.2Buffer
8、sofconstantionicstrength...........................................132.2ASSAYSFORACTIVITY.....................................................................152.2.1Enzymeassays.............................................