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ID:33013146
大小:3.30 MB
页数:49页
时间:2019-02-19
《快速和延迟性远程缺血后处理对大鼠脑缺血保护作用分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、andtheremoteischemicpostconditioningwasadministeredatthebeginningofreperfusionbywithdrawingmonofilamentsuture.(3)D-RIPGroup:themiddlecerebralarteryofratswasoccludedfor90min,andtheremoteischemicpostconditioningwasadministeredat6hoursafterreperfusionbywithdrawingmonofilamentsuture.Thebilateralfem
2、oralarteriesbluntlyseparated,atthebeginningofreperfusionor6hoursafterreperfusion,close/openthem10mininturnfor3circlesbyflogheartclip,namelyremoteischemicpostconditioning(RIP).Functionalneurologicaloutcomeweremeasuredat1hourafterocclusion,12hours,24houm48hoursafterreperfusion,respectively.Atlast
3、ratsweresacrificedat48hoursafterreperfusion,theparaffin-embeddedtissueweretakentheinfarctsizeweremeasuredbyHEstain.Secondly,Inordertoinvestigatetheunderlyingneuroprotectivemechanismofrapiddelayedremoteischemicpostconditioning(R/D--RIP)onischemiaitsdifferences·Weselected24malehealthySDratsthenra
4、ndomlydividedinto4groups,6ratspergroup:(1)ShamGroup;(2)ControlGroup;(3)R-RIPGroup;(4)D—RIPGroup·Ratsweresacrificedat48hoursafterreperfusion,thenthespecimensweretaken.Wecompletetherelevantcellmolecularbiologyindexdetection.Results:(1)TheinfarctvolumemeasuredbyHEstainingshowthatcomparedwiththecon
5、tr01.R-RIPD-RIPgroupweresignificantlysmaller(P<0.05);moreover,theinfarctvolumeofR-RIPgroupwasalsosignificantlysmallerthanR-RIPCgroupfP6、IPandD—RIPgroupweresignificantlyhigherthanthecontrol(P<0.05).Theneurologicalscoretestedat1afterocclusion,12hour,24hoursafterreperfusionofD—RIPgroupwaslowerthanR-RIPgroup,buttherewerenosignificantdifferentbetweenthetwogroups(P>0.05);howevertheneurologicalscoretestedat48hoursafterreperfusionofD—R7、IPgroupweresignificantlylowerthanR-RIPgroup(P<0.05).Conclusions:Ourresultsimplythatbothrapidanddelayedremoteischemicpostconditioning(wo-RiP)coulddecreasetheinfarctvolumeofischemiainrats,andimprovetheneurologicalscore;moreoverR-RIP
6、IPandD—RIPgroupweresignificantlyhigherthanthecontrol(P<0.05).Theneurologicalscoretestedat1afterocclusion,12hour,24hoursafterreperfusionofD—RIPgroupwaslowerthanR-RIPgroup,buttherewerenosignificantdifferentbetweenthetwogroups(P>0.05);howevertheneurologicalscoretestedat48hoursafterreperfusionofD—R
7、IPgroupweresignificantlylowerthanR-RIPgroup(P<0.05).Conclusions:Ourresultsimplythatbothrapidanddelayedremoteischemicpostconditioning(wo-RiP)coulddecreasetheinfarctvolumeofischemiainrats,andimprovetheneurologicalscore;moreoverR-RIP
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